Department of Biomedical Engineering, University of Michigan Medical School, Ann Arbor, MI, USA.
Department of Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
Biorheology. 2020;57(1):15-26. doi: 10.3233/BIR-190234.
Obesity-induced chronic inflammation and fibrosis in adipose tissue contributes to the progression of type 2 diabetes mellitus (DM). While fibrosis is known to induce mechanical stiffening of numerous tissue types, it is unknown whether DM is associated with alterations in adipose tissue mechanical properties.
The purpose of this study was to investigate whether DM is associated with differences in bulk viscoelastic properties of adipose tissue from diabetic (DM) and non-diabetic (NDM) obese subjects.
Bulk shear rheology was performed on visceral (VAT) and subcutaneous (SAT) adipose tissue, collected from obese subjects undergoing elective bariatric surgery. Rheology was also performed on the remaining extracellular matrix (ECM) from decellularized VAT (VAT ECM). Linear mixed models were used to assess whether correlations existed between adipose tissue mechanical properties and DM status, sex, age, and body mass index (BMI).
DM was not associated with significant differences in adipose tissue viscoelastic properties for any of the tissue types investigated. Tissue type dependent differences were however detected, with VAT having significantly lower shear storage and loss moduli than SAT and VAT ECM independent of DM status.
Although DM is typically associated with adipose tissue fibrosis, it is not associated with differences in macroscopic adipose tissue mechanical properties.
肥胖引起的脂肪组织慢性炎症和纤维化会导致 2 型糖尿病（DM）的进展。虽然众所周知纤维化会导致许多组织类型的机械僵硬，但尚不清楚 DM 是否与脂肪组织力学性能的改变有关。
本研究旨在探讨 DM 是否与肥胖的糖尿病（DM）和非糖尿病（NDM）患者的脂肪组织整体粘弹性特性存在差异有关。
对接受择期减肥手术的肥胖患者的内脏（VAT）和皮下（SAT）脂肪组织进行整体剪切流变学检测。还对脱细胞 VAT（VAT ECM）的剩余细胞外基质（ECM）进行流变学检测。线性混合模型用于评估脂肪组织力学特性与 DM 状态、性别、年龄和体重指数（BMI）之间是否存在相关性。
DM 与所研究的任何组织类型的脂肪组织粘弹性特性均无显著差异。然而，检测到了组织类型依赖性差异，VAT 的剪切储存和损耗模量明显低于 SAT 和 VAT ECM，与 DM 状态无关。
尽管 DM 通常与脂肪组织纤维化有关，但它与宏观脂肪组织力学性能的差异无关。
