Department of Biomedical Engineering, Key Laboratory of Ministry of Education, Zhejiang University, Hangzhou 310027, People's Republic of China. Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou 310027, People's Republic of China.
Biofabrication. 2020 Mar 13;12(2):025026. doi: 10.1088/1758-5090/ab782c.
Improving the proliferation of probiotics (ca. Bifidobacterium) and inhibiting the growth of pathogenic bacteria (ca. Escherichia coli) is crucial for human health. This study demonstrates the fabrication of core-shell structure fibers using electrohydrodynamic 3D printing to help improve gastrointestinal tract microbial content. These fibers have various geometries and are capable of encapsulating stachyose into cellulose acetate (shell layer) and loading proteoglycan into polyacrylic resin II (core layer). The impact of membrane geometry on drug release behavior and the effect of exchanging the loading site on physicochemical properties of the resulting fibers were studied. The printed fibrous membranes possess a biphasic drug release profile in simulated intestinal fluid with a burst release within the first 12 h and a slower sustained release up to 72 h. The speed order priority for drug release rate of the printed membrane was whole-circle > semi-circle > square. Moreover, the membranes exhibit good biocompatibility on L929 cells and excellent improvement effects on Bifidobacterium bifidum, combining inhibition effects on Escherichia coli. In summary, the dual-drug fibrous membranes presented here and their precision-fabricated patterns pave a new direction for improving the gastrointestinal tract microbial ecosystem health in the human body.
提高益生菌（双歧杆菌属）的增殖和抑制致病菌（大肠杆菌属）的生长对人类健康至关重要。本研究展示了使用静电纺丝 3D 打印来制造核壳结构纤维，以帮助改善胃肠道微生物含量。这些纤维具有各种几何形状，能够将棉子糖包封在醋酸纤维素（壳层）中，并将糖胺聚糖加载到聚丙烯酸树脂 II（芯层）中。研究了膜几何形状对药物释放行为的影响以及交换加载位置对所得纤维理化性质的影响。打印的纤维膜在模拟肠液中具有双相药物释放曲线，在前 12 小时内有突释，随后缓慢持续释放长达 72 小时。打印膜的药物释放速率的速度优先顺序为整圆>半圆>正方形。此外，这些膜在 L929 细胞上表现出良好的生物相容性，并对双歧杆菌有很好的改善效果，同时抑制大肠杆菌。总之，本文提出的双药物纤维膜及其精密制造的图案为改善人体胃肠道微生物生态系统健康开辟了新的方向。
