National Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, 38040, Pakistan.
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, 38040, Pakistan.
Probiotics Antimicrob Proteins. 2019 Sep;11(3):830-839. doi: 10.1007/s12602-017-9384-5.
Longevity of probiotic is the main concern for getting maximum benefits when added in food product. Bifidobacterium, a probiotic, tends to lose its viability during gastrointestinal track (GIT) transit and storage of food. Their viability can be enhanced through microencapsulation technology. In this study, Bifidobacterium bifidum (B. bifidum) ATCC 35914 was encapsulated by using two experimental plans. In the first plan, chitosan (CH) at 0.6, 0.8, and 1.0% and sodium alginate (SA) at 4, 5, and 6% were used. Based on encapsulation efficiency, 6% sodium alginate and 0.8% chitosan were selected for single coating of the bacteria, and the resulting micro beads were double coated with different concentrations (5, 7.5, and 10%) of whey protein concentrate (WPC) in the second plan. Encapsulation efficiency and GIT tolerance were determined by incubating the micro beads in simulated gastrointestinal juices (SIJ) at variable pH and exposure times, and their release (liberation of bacterial cells) profile was also observed in SIJ. The microencapsulated bacterial cells showed significantly (P < 0.01) higher viability as compared to the unencapsulated (free) cells during GIT assay. The double-coated micro beads SA 6%-WPC 5% and CH 0.8%-WPC 5% were proven to have the higher survival at pH 3.0 after 90 min of incubation time and at pH 7.0 after 3-h exposure in comparison to free cells in simulated conditions of the stomach and intestine, respectively. Moreover, double coating with whey protein concentrate played a significant role in the targeted (10 CFU/mL) delivery under simulated intestinal conditions.
益生菌的保质期是将其添加到食品中以获得最大益处的主要关注点。双歧杆菌是一种益生菌，在胃肠道（GIT）转运和食品储存过程中往往会失去其生存能力。通过微胶囊化技术可以提高其生存能力。在这项研究中，使用两种实验方案对双歧杆菌（Bifidobacterium bifidum）ATCC 35914 进行了包封。在第一个方案中，使用了 0.6%、0.8%和 1.0%的壳聚糖（CH）和 4%、5%和 6%的海藻酸钠（SA）。基于包封效率，选择 6%的海藻酸钠和 0.8%的壳聚糖对细菌进行单层包封，然后用不同浓度（5%、7.5%和 10%）的乳清蛋白浓缩物（WPC）对所得微球进行双层包封。通过在不同 pH 值和暴露时间的模拟胃肠道消化液（SIJ）中孵育微球，确定包封效率和 GIT 耐受性，并观察其在 SIJ 中的释放（细菌细胞的释放）情况。与未包封（游离）细胞相比，微胶囊化细菌细胞在 GIT 试验中表现出显著（P<0.01）更高的生存能力。与游离细胞相比，在 pH 3.0 孵育 90 分钟和 pH 7.0 暴露 3 小时后，在模拟胃和肠条件下，SA 6%-WPC 5%和 CH 0.8%-WPC 5%的双层包封微珠具有更高的存活率。此外，在模拟肠道条件下，乳清蛋白浓缩物的双层包封在靶向（10 CFU/mL）输送方面发挥了重要作用。
