Department of Bioengineering and Biotechnology, Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian 361021, People's Republic of China.
Department of Bioengineering and Biotechnology, Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian 361021, People's Republic of China.
Int J Biol Macromol. 2020 May 15;151:870-877. doi: 10.1016/j.ijbiomac.2020.02.195. Epub 2020 Feb 19.
A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana. The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM). A homogeneous polysaccharide JUYP was obtained with the yield of 21.2% and average molecular weight (Mw) of 577 kDa. Monosaccharide composition analysis indicated that JUYP was composed of glucose, galactose and mannose with a molar ratio of 2.3:1:0.7. Structural analyses demonstrated that the dominate components in JUYP were 6-β-D-Glcp, and other sugar residues included 2,4-β-D-Manp and T-β-D-Galf. TEM images further revealed JUYP was a linear branched molecule with entangled chains. Based on the anti-inflammatory assays, 1 μg/mL of JUYP exhibited good inhibitory effects on TNF-α, IL-6, IL-1β and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 cells, while the inhibitory effects (87.8% for mRNA, 55.89% for protein) of JUYP on IL-1β expressions were more significant than that of dexamethasone (DXMS, 61.6% for mRNA, 35.15% for protein) (p<0.01).
从卵状玉蕈中分离纯化得到一种多糖 JUYP。采用气相色谱（GC）、傅里叶变换红外光谱（FTIR）、甲基化 GC-MS、核磁共振（NMR）和透射电子显微镜（TEM）等方法对 JUYP 的结构进行了研究。得到了一种均一的多糖 JUYP，产率为 21.2%，平均分子量（Mw）为 577 kDa。单糖组成分析表明，JUYP 由葡萄糖、半乳糖和甘露糖组成，摩尔比为 2.3:1:0.7。结构分析表明，JUYP 的主要成分是 6-β-D-Glcp，其他糖残基包括 2,4-β-D-Manp 和 T-β-D-Galf。TEM 图像进一步表明 JUYP 是一种线性支化分子，链相互缠绕。基于抗炎实验，1μg/mL 的 JUYP 对 LPS 刺激的 RAW 264.7 细胞中 TNF-α、IL-6、IL-1β 和 COX-2 mRNA 的表达有很好的抑制作用，而 JUYP 对 IL-1β 表达的抑制作用（mRNA 为 87.8%，蛋白为 55.89%）比地塞米松（DXMS，mRNA 为 61.6%，蛋白为 35.15%）更为显著（p<0.01）。
