Department of Bioengineering and Biotechnology, Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, People's Republic of China.
PLoS One. 2019 Oct 4;14(10):e0223198. doi: 10.1371/journal.pone.0223198. eCollection 2019.
A novel polysaccharide STSP-I was isolated and purified from Sargassum thunbergii. Its structure and bioactivity were studied using gas chromatography (GC), fourier transform infrared spectroscopy (FTIR), periodate oxidation-smith degradation, partial acid hydrolysis, methylation-GC-MS, nuclear magnetic resonance (NMR), transmission electron microscopy (TEM), radicals scavenging assays and anti-inflammatory assays. STSP-I was consisted of fucose and galactose with a molar ratio of 1.2:1, and its mass was 373 kDa. The main structural components of STSP-I were →4)-α-D-Galp-(1→ and →3)-β-L-Fucp-(1→, STSP-I was a non-branched polysaccharide, and TEM further revealed the existence of entangled chains and linear forms. Compared with Vitamin C (Vc), STSP-I showed a higher scavenging effect of superoxide radical (EC50 = 0.22 mg/mL) and an equivalent scavenging effect of hydroxyl radical (EC50 = 0.88 mg/mL). STSP-I also exhibited good inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells, and the inhibitory effects were more than 91% at the concentrations of 75 and 150 μg/ml. The results indicate that the polysaccharide STSP-I from S. thunbergii with the linear structure may serve as potential antioxidant and anti-inflammatory agents.
一种新型多糖 STSP-I 从马尾藻中分离纯化。采用气相色谱（GC）、傅里叶变换红外光谱（FTIR）、高碘酸氧化-史密斯降解、部分酸水解、甲基化-GC-MS、核磁共振（NMR）、透射电子显微镜（TEM）、自由基清除实验和抗炎实验研究了其结构和生物活性。STSP-I 由岩藻糖和半乳糖组成，摩尔比为 1.2:1，分子量为 373 kDa。STSP-I 的主要结构组分为→4）-α-D-Galp-（1→和→3）-β-L-Fucp-（1→，STSP-I 是一种无支链多糖，TEM 进一步揭示了缠结链和线性形式的存在。与维生素 C（Vc）相比，STSP-I 对超氧自由基的清除效果更高（EC50=0.22mg/mL），对羟基自由基的清除效果相当（EC50=0.88mg/mL）。STSP-I 对 LPS 刺激的 RAW 264.7 小鼠巨噬细胞中 TNF-α、IL-6 和 COX-2 mRNA 表达也有较好的抑制作用，在 75 和 150μg/ml 浓度下抑制率均超过 91%。结果表明，具有线性结构的马尾藻多糖 STSP-I 可能作为潜在的抗氧化和抗炎剂。
