Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
Int J Biol Macromol. 2021 Jun 30;181:743-751. doi: 10.1016/j.ijbiomac.2021.03.176. Epub 2021 Mar 30.
In this study, an active component UP1-1 was isolated from Chinese Huangshan Umbilicaria esculenta via hot water extraction and purified by anion-exchange and gel-filtration chromatography. UP1-1 mainly composed of galactose, mannose and glucose in a molar ratio of 0.8:1.0:4.6 with an average molecular weight of 281 kDa. Methylation analysis of UP1-1 revealed the major glycosidic bonds comprised 1,6-linked Glcp, 1,4-linked Glcp, t-linked Glcp, 1,3,6-linked Manp, 1,3-linked Galp, t-linked Galp at the ratio of 2.28:0.38:0.32:0.63:0.25:0.29. Structural analysis results revealed that the backbone of UP1-1 consisted of →6)-β-D-Glcp-(1→, →6)-β-D-Manp-(1→, →4)-β-D-Glcp-(1 → residues with side chains of →3)-β-D-Galp-(1→, β-D-Galp-(1 → and β-D-Glcp-(1 → branches located at O-3 position of →6)-β-D-Manp-(1→. Immunostimulatory activity tests showed that UP1-1 could promote the phagocytic activity and NO production of RAW 264.7 cells in a dose-dependent manner. UP1-1 could significantly improve the proliferation effect of RAW 264.7 cells at the concentration of 50 μg/mL. Thus, UP1-1 exerted good immunostimulatory activity, suggesting that UP1-1 has a great potential application in pharmacological industry.
在这项研究中，通过热水提取从黄山产的皱脐乳菇中分离出活性成分 UP1-1，并用阴离子交换和凝胶过滤色谱法进行纯化。UP1-1 主要由半乳糖、甘露糖和葡萄糖组成，摩尔比为 0.8:1.0:4.6，平均分子量为 281 kDa。UP1-1 的甲基化分析表明，主要糖苷键由 1,6-连接的 Glcp、1,4-连接的 Glcp、t-连接的 Glcp、1,3,6-连接的 Manp、1,3-连接的 Galp 和 t-连接的 Galp 组成，比例为 2.28:0.38:0.32:0.63:0.25:0.29。结构分析结果表明，UP1-1 的骨架由→6)-β-D-Glcp-(1→、→6)-β-D-Manp-(1→、→4)-β-D-Glcp-(1 →残基和位于→3)-β-D-Galp-(1→、β-D-Galp-(1 →和β-D-Glcp-(1 →分支的侧链组成，位于→6)-β-D-Manp-(1→的 O-3 位。免疫刺激活性测试表明，UP1-1 可以剂量依赖性地促进 RAW 264.7 细胞的吞噬活性和 NO 产生。UP1-1 在 50 μg/mL 浓度下能显著改善 RAW 264.7 细胞的增殖效果。因此，UP1-1 表现出良好的免疫刺激活性，表明 UP1-1 在药理学工业中有很大的潜在应用价值。
