Cas3 蛋白——多面手的综述。

School of Life Sciences, University of Nottingham, Nottingham, NG7 2UH, UK.

Department of Biology, Faculty of Science, University of Zagreb, 10 000 Zagreb, Croatia.

Genes (Basel). 2020 Feb 18;11(2):208. doi: 10.3390/genes11020208.

Cas3 has essential functions in CRISPR immunity but its other activities and roles, in vitro and in cells, are less widely known. We offer a concise review of the latest understanding and questions arising from studies of Cas3 mechanism during CRISPR immunity, and highlight recent attempts at using Cas3 for genetic editing. We then spotlight involvement of Cas3 in other aspects of cell biology, for which understanding is lacking-these focus on CRISPR systems as regulators of cellular processes in addition to defense against mobile genetic elements.

Cas3 在 CRISPR 免疫中具有重要功能，但它在体外和细胞中的其他活性和作用尚未得到广泛了解。我们提供了对 Cas3 在 CRISPR 免疫过程中的机制研究的最新理解和出现的问题的简要回顾，并强调了最近利用 Cas3 进行基因编辑的尝试。然后，我们关注 Cas3 在细胞生物学其他方面的参与，对于这些方面，我们的理解还很缺乏——这些方面的重点是 CRISPR 系统作为除了抵御移动遗传元件之外调节细胞过程的调节剂。