Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, Jiangsu, China.
Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Int J Mol Sci. 2020 Feb 18;21(4):1371. doi: 10.3390/ijms21041371.
Hyperbaric oxygen (HBO) is widely applied to treat several hypoxia-related diseases. Previous studies have focused on the immediate effect of HBO-exposure induced oxidative stress on the lungs, but knowledge regarding the chronic effects from repetitive HBO exposure is limited, especially at the gene expression level. We found that repetitive HBO exposure did not alter the morphology of murine lungs. However, by deconvolution of RNA-seq from those mice lungs using CIBERSORTx and the expression profile matrices of 8 mesenchymal cell subtypes obtained from bleomycin-treated mouse lungs, we identify several mesenchymal cell subtype changes. These include increases in matrix fibroblasts, mesenchymal progenitors and mesothelial cell populations and decreases in lipofibroblasts, endothelial and high cell populations. Our data suggest that repetitive HBO exposure may affect biological processes in the lungs such as response to wounding, extracellular matrix, vasculature development and immune response.
高压氧（HBO）被广泛应用于治疗多种与缺氧相关的疾病。先前的研究主要集中在 HBO 暴露引起的氧化应激对肺部的即时影响上，但对于重复 HBO 暴露的慢性影响知之甚少，尤其是在基因表达水平上。我们发现重复 HBO 暴露不会改变小鼠肺部的形态。然而，通过使用 CIBERSORTx 对来自这些小鼠肺部的 RNA-seq 进行去卷积，并使用博来霉素处理的小鼠肺部获得的 8 种间充质细胞亚型的表达谱矩阵，我们鉴定出几种间充质细胞亚型的变化。这些变化包括基质成纤维细胞、间充质祖细胞和间皮细胞群体的增加，以及脂肪成纤维细胞、内皮细胞和高细胞群体的减少。我们的数据表明，重复 HBO 暴露可能会影响肺部的生物学过程，如对创伤的反应、细胞外基质、血管生成和免疫反应。
