Laboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas s/n, Col. Haciendita, 39070, Chilpancingo, Guerrero, México.
Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas s/n, Col. Haciendita, 39070, Chilpancingo, Guerrero, México.
Gastric Cancer. 2020 Jul;23(4):754-759. doi: 10.1007/s10120-020-01049-5. Epub 2020 Feb 21.
In women, serum levels of CTSB, GKN2, LIPF, LIPFG, AZGP1, TOP2A and PGA4 are proposed as predictive markers of gastric cancer. It is unknown whether GKN1 expression varies with the sex of patients with chronic gastritis or gastric cancer. We studied 36 patients with histopathological diagnosis of chronic gastritis from the state of Guerrero, Mexico. PCR was performed for H. pylori detection and GKN1 expression was determined by RT-qPCR and western blot. GKN1 mRNA expression was significantly lower in patients with chronic follicular gastritis than in those with chronic chemical gastritis (p = 0.00071). The mRNA and protein level of expression of GKN1 were significantly lower in women with chronic follicular gastritis than in men with the same condition (p = 0.0279 and p = 0.0014, respectively); the lowest levels of GKN1 were detected in women with H. pylori-positive follicular gastritis (p = 0.0175 and p = 0.0111, respectively). Through a bioinformatic analysis, estrogen response elements were identified in the GKN1 promoter.
在女性中，CTSB、GKN2、LIPF、LIPFG、AZGP1、TOP2A 和 PGA4 的血清水平被提议作为胃癌的预测标志物。尚不清楚慢性胃炎或胃癌患者的 GKN1 表达是否随性别而变化。我们研究了来自墨西哥格雷罗州的 36 名经组织病理学诊断为慢性胃炎的患者。进行了 PCR 检测以检测 H. pylori，并通过 RT-qPCR 和 Western blot 确定了 GKN1 的表达。慢性滤泡性胃炎患者的 GKN1 mRNA 表达明显低于慢性化学性胃炎患者（p=0.00071）。慢性滤泡性胃炎女性的 GKN1 mRNA 和蛋白表达水平明显低于同条件的男性（p=0.0279 和 p=0.0014）；在 H. pylori 阳性滤泡性胃炎的女性中检测到的 GKN1 水平最低（p=0.0175 和 p=0.0111）。通过生物信息学分析，在 GKN1 启动子中鉴定出了雌激素反应元件。
