Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia.
J Biomed Mater Res B Appl Biomater. 2020 Aug;108(6):2560-2570. doi: 10.1002/jbm.b.34587. Epub 2020 Feb 22.
Burns and chronic wounds are especially challenging wounds to heal. In efforts to heal these wounds, physicians often use autologous skin grafts to help restore mechanical and barrier functionality to the wound area. These grafts are, by nature, limited in availability. In an effort to provide an alternative, we have developed an electrospun wound dressing designed to incorporate into the wound with the option to deliver a cellular payload. Here, a blend of poly(glycolic acid) and poly(ethylene glycol) was electrospun as part of a custom fabrication method that incorporated 3D printed poly(vinyl alcohol) sacrificial elements. This preparation is unique compared to traditional electrospinning as sacrificial elements provide an internal void space for an injectable payload to be delivered to the wound site. When the construct was tested in vivo (full thickness excisional skin wounds), wound closure was slightly delayed by the presence of the scaffold in both normal and challenged wounds. Quality of healing was improved in normal wounds as measured by histomorphometrics when treated with the construct and exhibited increased neovascularization. Our results demonstrate that the extracellular matrix-like scaffold developed in this study is beneficial to healing of full thickness skin defects and may benefit challenged wounds.
烧伤和慢性创面是特别具有挑战性的愈合创面。为了治愈这些创面，医生通常使用自体皮片移植来帮助恢复创面的机械和屏障功能。这些移植物的供应本身就受到限制。为了提供一种替代方法，我们开发了一种静电纺丝创面敷料，旨在与创面结合，并可选择输送细胞有效载荷。在这里，聚（乙醇酸）和聚（乙二醇）的混合物被静电纺丝，作为一种定制制造方法的一部分，该方法结合了 3D 打印的聚乙烯醇牺牲元件。与传统静电纺丝相比，这种制备方法是独特的，因为牺牲元件为可注射有效载荷提供了一个内部的中空空间，以便输送到创面部位。当该构建体在体内（全层切除皮肤创面）进行测试时，在正常和挑战性创面中，支架的存在略微延迟了创面的闭合。当用该构建体治疗时，正常创面的愈合质量通过组织形态计量学得到改善，表现出增加的新生血管化。我们的结果表明，本研究中开发的细胞外基质样支架有利于全层皮肤缺损的愈合，并可能有益于挑战性创面。
