1 Biomaterials Research Center, Korea Institute of Science and Technology , Seoul, Republic of Korea.
2 KU-KIST Graduate School of Converging Science and Technology, Korea University , Seoul, Republic of Korea.
Tissue Eng Part A. 2018 May;24(9-10):830-848. doi: 10.1089/ten.TEA.2017.0318. Epub 2018 Jan 9.
Produced through electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties as follows: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration. In particular, heart decellularized ECM (hdECM) has various pro-angiogenic factors that can induce angiogenesis for wound healing. In this regard, a nanofibrous electrospun hdECM-based hybrid scaffold (NEhdHS), which is a PLCL membrane, including hdECM as an active agent, was tested as a wound dressing to assess its fundamental biochemical and physical features in wound healing. Use of NEhdHS with its porous structure and pro-angiogenic factors is expected to provide an effective wound dressing and reduced scarring. We first demonstrate the effectiveness of a proposed decellularization protocol through analysis of dECM components and describe the mechanical properties of the fabricated NEhdHS. Next, we present an in vitro angiogenesis analysis of the NEhdHS, using a coculture system with human dermal fibroblasts and human umbilical vein endothelial cells; the results of which confirm its biocompatibility and show that the NEhdHS can significantly enhance angiogenesis over that obtained from PLCL or gelatin-containing PLCL scaffolds. We also studied the effectiveness of the NEhdHS in vivo. Using a rat excisional wound-splinting model, we show that covering the upper part of the wound with NEhdHS significantly reduces scarring in the wound healing process compared to that with PLCL or gelatin-containing PLCL scaffolds. Based upon its properties, we conclude that the NEhdHS has potential for application in wound dressing.
通过静电纺丝制备的聚（L-丙交酯-共-己内酯）（PLCL）膜具有多孔结构和可生物降解性，在各个医学领域都有应用。多孔结构的静电纺丝膜具有以下几个有利特性，因此特别有趣：它能从伤口渗出液体，不会在伤口覆盖物下积聚，也不会导致伤口干燥。此外，通过组织脱细胞化获得的细胞外基质（ECM）结构可用作工程化组织支架和细胞再生的支撑物。特别是，去细胞化的心脏 ECM（hdECM）具有多种促血管生成因子，可诱导血管生成以促进伤口愈合。在这方面，我们测试了一种基于 hdECM 的纳米纤维静电纺丝杂化支架（NEhdHS）作为伤口敷料，以评估其在伤口愈合中的基本生化和物理特性。使用具有多孔结构和促血管生成因子的 NEhdHS 有望提供有效的伤口敷料并减少疤痕形成。我们首先通过分析 dECM 成分来证明所提出的脱细胞化方案的有效性，并描述了所制备的 NEhdHS 的机械性能。接下来，我们使用人真皮成纤维细胞和人脐静脉内皮细胞的共培养系统对 NEhdHS 的体外血管生成进行了分析；结果证实了其生物相容性，并表明 NEhdHS 可显著增强血管生成，超过了 PLCL 或含明胶的 PLCL 支架。我们还研究了 NEhdHS 在体内的有效性。使用大鼠切除性伤口夹板模型，我们表明在伤口愈合过程中，用 NEhdHS 覆盖伤口的上部可显著减少疤痕形成，与用 PLCL 或含明胶的 PLCL 支架相比。基于其特性，我们得出结论，NEhdHS 具有在伤口敷料中应用的潜力。
