Oncology Graduate Program, Molecular Carcinogenesis Program, Research Center, Brazilian National Cancer Institute. Rua André Cavalcanti, 37, Centro, 20231-050, Rio de Janeiro, Brazil.
Molecular Carcinogenesis Program, Brazilian National Cancer Institute. Rua André Cavalcanti, 37, Centro, 20231-050, Rio de Janeiro, Brazil.
Cancer Lett. 2020 May 1;477:70-75. doi: 10.1016/j.canlet.2020.02.023. Epub 2020 Feb 19.
Oropharyngeal squamous cell carcinoma (OSCC) is a fatal and highly incident disease. Although tobacco and alcohol consumption are the main risk factors associated with OSCC, a recent significant increase in OSCC HPV16 positive cases in high-income countries has been observed. However, it is not clear whether this change is also present in low- and middle-income countries. In this study, we evaluated HPV16 prevalence in 346 OSCC cases diagnosed in the largest Brazilian oncology public hospital by using the combination of two techniques, HPV16 E6 detection by qPCR and p16 immunohistochemistry. In total, 11.9% of cases were HPV16 E6 positive, 9.2% were p16 positive and 6.1% were positive in both analyses. There was a predominance of keratinizing-SCC, with only four HPV-positive cases showing basaloid-like or non-keratinizing-SCC. HPV infection had no impact on disease-free or overall survival, while alcohol use was an independent prognostic factor for overall survival. Most cases reported a high frequency of tobacco (94.6%) and alcohol consumption (88.2%), were of low education level, and typically presented at advanced clinical stages, indicating that the profile of Brazilian OSCC patients has not changed.
口咽鳞状细胞癌（OSCC）是一种致命且高发的疾病。尽管吸烟和饮酒是与 OSCC 相关的主要风险因素，但最近在高收入国家观察到 HPV16 阳性的 OSCC 病例显著增加。然而，这种变化是否也存在于中低收入国家尚不清楚。在这项研究中，我们通过使用两种技术的组合，即 HPV16 E6 的 qPCR 检测和 p16 免疫组化，评估了巴西最大的肿瘤学公立医院诊断的 346 例 OSCC 病例中 HPV16 的流行率。总共，11.9%的病例 HPV16 E6 阳性，9.2%的病例 p16 阳性，6.1%的病例两种分析均为阳性。角化型 SCC 占主导地位，只有 4 例 HPV 阳性病例表现为基底样或非角化型 SCC。HPV 感染对无病生存期或总生存期没有影响，而饮酒是总生存期的独立预后因素。大多数病例报告吸烟（94.6%）和饮酒（88.2%）频率高，受教育程度低，通常处于晚期临床阶段，表明巴西 OSCC 患者的特征没有改变。
