洋葱对翼腭动脉（PPA）结扎小鼠的视网膜神经节细胞具有神经保护作用。
Allium cepa exerts neuroprotective effect on retinal ganglion cells of pterygopalatine artery (PPA) ligated mice.
作者信息
Kumar Saurabh, Modgil Shweta, Bammidi Sridhar, Minhas Gillipsie, Shri Richa, Kaushik Sushmita, Singh Varinder, Anand Akshay
机构信息
Neuroscience Research Lab, Department of Neurology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, 160012, India.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147002, India.
出版信息
J Ayurveda Integr Med. 2020 Oct-Dec;11(4):489-494. doi: 10.1016/j.jaim.2019.08.002. Epub 2020 Feb 20.
BACKGROUND
Repeated failure to rescue the damaged retinal ganglion cells (RGCs) by various drugs has warranted the need to screen common herbal compounds available in the form of various eye formulations for their efficacy.
OBJECTIVE
We aimed to investigate the neuroprotective effect of pretreatment with aqueous extract of A. cepa in Ischemia/Reperfusion (I/R) induced retinal injury.
METHODS
Ischemia was induced for 2 h by pterygopalatine artery (PPA) ligation in C57BL/6J mice, followed by reperfusion. The neuroprotective role of oral pretreatment with aqueous extract of A. cepa (300 mg/kg) was analyzed with respect to control and injury only group at 7, 14, and 28 day after the surgery for expression of different genes in the retina by Real-Time PCR.
RESULTS
Molecular analysis at different time points showed increased expression of BCl-2, GDNF, GFAP, and Brn3b in the retina at 14 and 28 day after A. cepa treatment in comparison to the injury alone group. However, at shorter time point (7th day), the expression of these genes was pronounced in the injury only group in comparison to the injury and pretreated group.
CONCLUSION
Pretreatment with aqueous extract of A. cepa may protect from the neuronal damage in I/R-induced retinal injury in mice by altering the expression of neurotrophic factor.
背景
多种药物反复未能挽救受损的视网膜神经节细胞（RGCs），这使得有必要筛选各种眼部制剂形式的常见草药化合物的功效。
目的
我们旨在研究用洋葱水提取物预处理对缺血/再灌注（I/R）诱导的视网膜损伤的神经保护作用。
方法
通过结扎C57BL/6J小鼠的翼腭动脉（PPA）诱导缺血2小时，随后进行再灌注。在手术后7、14和28天，通过实时聚合酶链反应分析用洋葱水提取物（300毫克/千克）口服预处理相对于对照组和仅损伤组在视网膜中不同基因表达的神经保护作用。
结果
不同时间点的分子分析显示，与仅损伤组相比，在洋葱处理后14天和28天，视网膜中BCl-2、胶质细胞源性神经营养因子（GDNF）、胶质纤维酸性蛋白（GFAP）和Brn3b的表达增加。然而，在较短时间点（第7天），与损伤组和预处理组相比，这些基因在仅损伤组中的表达更为明显。
结论
用洋葱水提取物预处理可能通过改变神经营养因子的表达来保护小鼠免受I/R诱导的视网膜损伤中的神经元损伤。
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