BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Department of Genetics and Biotechnology, Vila Real, Portugal.
BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Department of Genetics and Biotechnology, Vila Real, Portugal.
Food Chem. 2020 Jul 1;317:126417. doi: 10.1016/j.foodchem.2020.126417. Epub 2020 Feb 15.
The detection of latent Colletotrichum spp infection in olive drupes is crucial, to avoid contamination in the olive oil production chain. In order to detect the presence of C. acutatum in complex olive matrices a real-time PCR assay was developed, using olive drupe and oil samples from C. acutatum susceptible and tolerant olive cultivars (Galega Vulgar, Cobrançosa and Picual) with different infection levels. A C. acutatum specific sequence, belonging to the Internal Transcribed Spacers region, was used to design the real-time PCR detection assay, resulting in an 490 bp amplicon with a consistent melting temperature (T = 87.8 °C). The assay allowed a rapid and high-sensitive C. acutatum detection mean, being able to detect the infection in a latent phase, for the first time, in olive drupes, 16 hai, and in olive oils containing 20% of infected olives. This novel method can be used to monitor C. acutatum presence in olive orchards.
检测橄榄中的潜伏性炭疽菌感染至关重要，可避免橄榄油生产链受到污染。为了在复杂的橄榄基质中检测到 C. acutatum 的存在，我们开发了一种实时 PCR 检测方法，使用来自易感性和耐受性橄榄品种（Galega Vulgar、Cobrançosa 和 Picual）的橄榄核和油样，这些品种的感染水平不同。我们使用属于内转录间隔区的 C. acutatum 特异性序列来设计实时 PCR 检测方法，得到一个 490 bp 的扩增子，其熔解温度（T）一致为 87.8°C。该方法可以快速、灵敏地检测 C. acutatum，首次在 16 公顷的橄榄核和含有 20%感染橄榄的橄榄油中检测到潜伏期感染。这种新方法可用于监测橄榄园中 C. acutatum 的存在。
