Departamento de Agronomía, ETSIAM, Universidad de Córdoba (UCO), Córdoba, Spain.
Department of Plant Pathology, University of California, Davis, Kearney Agricultural Research and Extension Center, Parlier, CA, USA.
J Appl Microbiol. 2018 Jan;124(1):209-219. doi: 10.1111/jam.13610. Epub 2017 Dec 11.
To set up a practical method to detect latent infections of Colletotrichum sp., the causal agent of olive anthracnose, on olives before the onset of disease symptoms.
Freezing, sodium hydroxide (NaOH), ethanol and ethylene treatments were evaluated to detect latent infections on inoculated and naturally infected olive fruit by Colletotrichum sp. as non-hazardous alternatives to paraquat. Treatments were conducted using fruit of cultivars Arbequina and Hojiblanca. The disease incidence and T were calculated. Dipping in NaOH 0·05% solution and the paraquat method were the most effective treatments on both inoculated and naturally infected fruit, although the value of T was lower for the NaOH method than for the paraquat method in one of the experiments. Subsequently, the dipping time in NaOH 0·05% was evaluated. Longer dipping times in NaOH 0·05% were better than shorter ones in cultivar Arbequina, with 72 h being the most effective in cultivar Hojiblanca.
NaOH solution is a practical method to detect latent infections of Colletotrichum sp. on immature olive fruit.
This study is relevant because we set up a viable, non-hazardous alternative to paraquat to detect latent infections of Colletotrichum sp. using NaOH. The use of NaOH is a simple and eco-friendly tool that allows the determination of the level of latent infections by Colletotrichum in olives. Therefore, our method will be useful in decision-making processes for disease management before the appearance of the first visible symptoms.
建立一种实用的方法来检测潜伏感染的 Colletotrichum sp.，即引起橄榄炭疽病的病原体，在疾病症状出现之前的橄榄上。
评估了冷冻、氢氧化钠 (NaOH)、乙醇和乙烯处理，以检测 Colletotrichum sp. 接种和自然感染的橄榄果实中的潜伏感染，作为替代百草枯的非危险方法。使用 Arbequina 和 Hojiblanca 品种的果实进行了处理。计算了疾病发病率和 T 值。在接种和自然感染的果实上，NaOH 0·05%溶液浸泡和百草枯处理是最有效的方法，尽管在一项实验中，NaOH 方法的 T 值比百草枯方法低。随后，评估了在 NaOH 0·05%溶液中的浸泡时间。在 Arbequina 品种中，较长的 NaOH 0·05%浸泡时间优于较短的浸泡时间，在 Hojiblanca 品种中，72 小时是最有效的。
NaOH 溶液是检测未成熟橄榄果实中 Colletotrichum sp. 潜伏感染的实用方法。
这项研究很重要，因为我们建立了一种可行的、非危险的替代百草枯的方法，使用 NaOH 来检测 Colletotrichum sp. 的潜伏感染。NaOH 的使用是一种简单且环保的工具，可以通过 Colletotrichum 在橄榄中的潜伏感染程度。因此，我们的方法将在出现第一个可见症状之前的疾病管理决策过程中非常有用。
