Gomes Sónia, Prieto Pilar, Martins-Lopes Paula, Carvalho Teresa, Martin Antonio, Guedes-Pinto Henrique
Institute of Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), Vila Real, Portugal.
Mycopathologia. 2009 Oct;168(4):203-11. doi: 10.1007/s11046-009-9211-y. Epub 2009 May 30.
Colletotrichum acutatum is a cosmopolitan and damaging plant pathogen of temperate, subtropical, and tropical fruits and causes anthracnose on olive (Olea europaea L.). Three olive cultivars showing a variable response to infection by C. acutatum were selected to a preliminary study of pathogen development. Fruit samples, from susceptible and tolerant cultivars, were taken at 0, 24, 48, 72, and 192 h after inoculation for a microscopic and histological study of the infection and colonization process. The aim of this study was to compare the infection process: conidial germination, germ tube and appressorium formation, hyphal growth, and mesocarp colonization in susceptible and tolerant olive cultivars as a condition for further exploration of disease development, which is required to develop cultivars with improved resistance to anthracnose. The rate of mesocarp colonization differed between the susceptible and tolerant cultivars, and both intracellular hemibiotrophy and subcuticular intramural necrotrophy were observed. Hemibiotrophic infection predominated in the moderately tolerant cultivar.
尖孢炭疽菌是一种广泛分布且具危害性的植物病原菌，可侵染温带、亚热带和热带水果，能引发油橄榄（Olea europaea L.）炭疽病。选取了对尖孢炭疽菌感染反应各异的三个油橄榄品种，对病原菌发育进行初步研究。在接种后0、24、48、72和192小时，采集感病和抗病品种的果实样本，用于对感染和定殖过程进行微观和组织学研究。本研究的目的是比较感病和抗病油橄榄品种的感染过程：分生孢子萌发、芽管和附着胞形成、菌丝生长以及中果皮定殖情况，以此作为进一步探究病害发展的条件，而这对于培育抗炭疽病能力更强的品种是必要的。感病和抗病品种中果皮定殖速率存在差异，同时观察到细胞内半活体营养型和皮下壁内坏死营养型两种情况。在中度抗病品种中，半活体营养型感染占主导。
