Milanović Vesna, Cardinali Federica, Aquilanti Lucia, Garofalo Cristiana, Roncolini Andrea, Sabbatini Riccardo, Clementi Francesca, Osimani Andrea
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Indian J Microbiol. 2020 Mar;60(1):115-118. doi: 10.1007/s12088-019-00817-x. Epub 2019 Aug 3.
The present study was aimed to get an insight into the bacterial biota of ready-to-eat small crickets () already marketed in the European Union. 16S rRNA gene of the DNAs extracted from thirty-two samples of ready-to-eat crickets commercialized by 4 European Union producers located in Austria, Belgium, France and the Netherlands (2 batches per producer) was analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The species belonging to the genera , and were detected in samples from Austria, while those from genera , and were detected in samples from Belgium. Moreover, samples from France were characterized by , , and genera. Finally, the genera , , and were identified in the samples produced in the Netherlands. When insects are intended for commercialization, rearing, processing and handling could affect the presence of the occurring microbial species. Hence, to assure a safe product, the need for a full standardization of production technologies, including feed supply as well as rearing and processing practices, is recommended.
本研究旨在深入了解欧盟市场上已销售的即食小蟋蟀的细菌群落。对位于奥地利、比利时、法国和荷兰的4家欧盟生产商商业化的32份即食蟋蟀样本(每家生产商2批次)提取的DNA的16S rRNA基因,通过聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)进行分析。在奥地利的样本中检测到了属于、和属的物种,而在比利时的样本中检测到了来自、和属的物种。此外,法国的样本以、、和属为特征。最后,在荷兰生产的样本中鉴定出了、、和属。当昆虫用于商业化时,饲养、加工和处理可能会影响所出现的微生物种类的存在。因此,为确保产品安全,建议对生产技术进行全面标准化,包括饲料供应以及饲养和加工操作。