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本文引用的文献

1
Current knowledge on the microbiota of edible insects intended for human consumption: A state-of-the-art review.人类食用的可食用昆虫的微生物组学研究进展：综述。
Food Res Int. 2019 Nov;125:108527. doi: 10.1016/j.foodres.2019.108527. Epub 2019 Jun 25.
2
Modulation of intestinal microbiota, morphology and mucin composition by dietary insect meal inclusion in free-range chickens.在放养肉鸡日粮中添加昆虫粉对肠道微生物群、形态和粘蛋白组成的调节作用。
BMC Vet Res. 2018 Dec 4;14(1):383. doi: 10.1186/s12917-018-1690-y.
3
The bacterial biota of laboratory-reared edible mealworms (Tenebrio molitor L.): From feed to frass.实验室饲养的食用黄粉虫（Tenebrio molitor L.）的细菌生物群：从饲料到粪便。
Int J Food Microbiol. 2018 May 2;272:49-60. doi: 10.1016/j.ijfoodmicro.2018.03.001. Epub 2018 Mar 7.
4
Microbial dynamics during production of lesser mealworms (Alphitobius diaperinus) for human consumption at industrial scale.工业化规模生产供人类食用的黄粉虫（Alphitobius diaperinus）过程中的微生物动态变化。
Food Microbiol. 2018 Apr;70:181-191. doi: 10.1016/j.fm.2017.09.012. Epub 2017 Sep 21.
5
Clostridium difficile and Clostridium sordellii toxins, proinflammatory versus anti-inflammatory response.艰难梭菌和索氏梭菌毒素，促炎反应与抗炎反应
Toxicon. 2018 Jul;149:54-64. doi: 10.1016/j.toxicon.2017.11.003. Epub 2017 Nov 14.
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Study of the bacterial diversity of foods: PCR-DGGE versus LH-PCR.食品细菌多样性研究：聚合酶链式反应-变性梯度凝胶电泳法与连接介导的聚合酶链式反应法的比较
Int J Food Microbiol. 2017 Feb 2;242:24-36. doi: 10.1016/j.ijfoodmicro.2016.11.008. Epub 2016 Nov 10.
7
Safety aspects of the production of foods and food ingredients from insects.昆虫源食品及食品成分生产的安全性问题。
Mol Nutr Food Res. 2017 Jun;61(6). doi: 10.1002/mnfr.201600520. Epub 2016 Nov 14.
8
Identification of chicken intestinal microbiota correlated with the efficiency of energy extraction from feed.鉴定与鸡从饲料中提取能量效率相关的肠道微生物群。
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9
Hespellia stercorisuis gen. nov., sp. nov. and Hespellia porcina sp. nov., isolated from swine manure storage pits.猪粪赫斯佩利亚菌属新属、新种及猪赫斯佩利亚菌新种，从猪粪储存坑中分离得到。
Int J Syst Evol Microbiol. 2004 Jan;54(Pt 1):241-245. doi: 10.1099/ijs.0.02719-0.

市售即食蟋蟀微生物群一瞥（）

A Glimpse into the Microbiota of Marketed Ready-to-Eat Crickets ().

作者信息

Milanović Vesna, Cardinali Federica, Aquilanti Lucia, Garofalo Cristiana, Roncolini Andrea, Sabbatini Riccardo, Clementi Francesca, Osimani Andrea

机构信息

Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.

出版信息

Indian J Microbiol. 2020 Mar;60(1):115-118. doi: 10.1007/s12088-019-00817-x. Epub 2019 Aug 3.

DOI:10.1007/s12088-019-00817-x
PMID:32089581
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7000585/
Abstract

The present study was aimed to get an insight into the bacterial biota of ready-to-eat small crickets () already marketed in the European Union. 16S rRNA gene of the DNAs extracted from thirty-two samples of ready-to-eat crickets commercialized by 4 European Union producers located in Austria, Belgium, France and the Netherlands (2 batches per producer) was analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The species belonging to the genera , and were detected in samples from Austria, while those from genera , and were detected in samples from Belgium. Moreover, samples from France were characterized by , , and genera. Finally, the genera , , and were identified in the samples produced in the Netherlands. When insects are intended for commercialization, rearing, processing and handling could affect the presence of the occurring microbial species. Hence, to assure a safe product, the need for a full standardization of production technologies, including feed supply as well as rearing and processing practices, is recommended.

摘要

本研究旨在深入了解欧盟市场上已销售的即食小蟋蟀的细菌群落。对位于奥地利、比利时、法国和荷兰的4家欧盟生产商商业化的32份即食蟋蟀样本（每家生产商2批次）提取的DNA的16S rRNA基因，通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）进行分析。在奥地利的样本中检测到了属于、和属的物种，而在比利时的样本中检测到了来自、和属的物种。此外，法国的样本以、、和属为特征。最后，在荷兰生产的样本中鉴定出了、、和属。当昆虫用于商业化时，饲养、加工和处理可能会影响所出现的微生物种类的存在。因此，为确保产品安全，建议对生产技术进行全面标准化，包括饲料供应以及饲养和加工操作。