Mitchaothai Jamlong, Grabowski Nils T, Lertpatarakomol Rachakris, Trairatapiwan Tassanee, Lukkananukool Achara
Office of Administrative Interdisciplinary Program on Agricultural Technology, School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok 10520, Thailand.
Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover (TiHo), 30173 Hannover, Germany.
Vet Sci. 2024 Jul 1;11(7):295. doi: 10.3390/vetsci11070295.
Food safety for cricket production is a crucial factor in producing edible crickets with safety for consumers and sustainability for two-spotted () as well as house () cricket production. This study was conducted by simultaneously rearing two cricket species, comprising two-spotted crickets () and house crickets (). A total of 16 rearing crates were used for the present study, which were allocated into 8 rearing crates for each studied cricket species, including paper egg cartons. Cricket eggs were incubated in the rearing crates. Once the crickets hatched, tap water and powdered feed were provided ad libitum throughout the experiment. At the end of this study (35 and 42 days for the two-spotted and house crickets, respectively), all crickets were harvested, rinsed in tap water, and boiled in water for 5 min. During the rearing and harvesting processes, samples were collected from various potential contamination points for bacteria, including and spp. There were samples of the initial input (feed, drinking water, and staff hands), rearing environment (water pipe, crate wall, living cartons, frass, and cricket surface), and harvesting crickets (harvested, washed, and boiled crickets), with a 2-week sampling interval, except for the last round of sampling for the two-spotted crickets. Subsequently, all samples were submitted to isolate and identify contaminated bacteria. The samples from the last round of sampling for both kinds of crickets were submitted to quantify the level of contamination for and spp., including antimicrobial resistance by the disk diffusion method for the positive isolate. The results showed that bacterial contamination was found in the rearing of both cricket species, primarily involving spp. and spp., mainly found in prepared drinking water and the water pipes of drinking water supply equipment, which are potential sources of contamination with cricket frass. was found in 4.8% and 4.3% of the two-spotted and house crickets, respectively, while no presence of spp. was detected in any submitted samples. The quantification of and spp. indicated contamination near the water pipe and the frass of two-spotted crickets, but spp. was undetectable in both two-spotted and house crickets. The antimicrobial resistance of isolated mainly involved penicillin G, amoxicillin, ampicillin, erythromycin, lincomycin, and tiamulin. Thus, good farm management with proper sanitation practices (such as cleaning and keeping the environment dry), as well as boiling crickets during the harvesting process, may help ensure the safety of edible cricket production.
蟋蟀生产的食品安全是生产对消费者安全且对双斑蟋以及家蟋生产具有可持续性的可食用蟋蟀的关键因素。本研究通过同时饲养两种蟋蟀进行，这两种蟋蟀分别是双斑蟋和家蟋。本研究共使用了16个饲养箱，每种被研究的蟋蟀物种分配8个饲养箱，包括纸制蛋托。蟋蟀卵在饲养箱中孵化。蟋蟀孵化后，在整个实验过程中自由提供自来水和粉状饲料。在本研究结束时（双斑蟋和家蟋分别为35天和42天），所有蟋蟀被收获，用自来水冲洗，并在水中煮5分钟。在饲养和收获过程中，从包括大肠杆菌和沙门氏菌属等各种潜在污染点采集细菌样本。有初始投入物（饲料、饮用水和工作人员手部）、饲养环境（水管、饲养箱壁、生活纸箱、粪便和蟋蟀体表）以及收获的蟋蟀（收获、清洗和煮熟的蟋蟀）的样本，采样间隔为2周，但双斑蟋的最后一轮采样除外。随后，所有样本被送去分离和鉴定污染细菌。两种蟋蟀最后一轮采样的样本被送去定量大肠杆菌和沙门氏菌属的污染水平，包括通过纸片扩散法对阳性分离株进行抗菌药物敏感性检测。结果表明，两种蟋蟀的饲养过程中均发现了细菌污染，主要涉及大肠杆菌属和沙门氏菌属，主要存在于制备好的饮用水和饮用水供应设备的水管中，这些是蟋蟀粪便污染的潜在来源。双斑蟋和家蟋中分别有4.8%和4.3%检测到大肠杆菌，而在任何提交的样本中均未检测到沙门氏菌属。大肠杆菌和沙门氏菌属的定量分析表明，双斑蟋的水管附近和粪便中有大肠杆菌污染，但双斑蟋和家蟋中均未检测到沙门氏菌属。分离出的大肠杆菌的抗菌药物敏感性主要涉及青霉素G、阿莫西林、氨苄西林、红霉素、林可霉素和泰妙菌素。因此，良好的农场管理以及适当的卫生措施（如清洁和保持环境干燥），以及在收获过程中煮蟋蟀，可能有助于确保可食用蟋蟀生产的安全性。
