Jeon Hyelim, Kim Wanhui, Kim Boyoung, Lee Sookyeong, Jayaraman Jay, Jung Gayoung, Choi Sera, Sohn Kee Hoon, Segonzac Cécile
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Plant Immunity Research Center, Seoul National University, Seoul 08826, Korea.
Plant Pathol J. 2020 Feb;36(1):43-53. doi: 10.5423/PPJ.OA.08.2019.0227. Epub 2020 Feb 1.
() is a causal agent of bacterial wilt in crops worldwide including Republic of Korea. virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both and , only one was dependent on suppressor of G2 allele of skp1 (SGT1), a molecular chaperone of nucleotide-binding and leucine-rich repeat immune receptors. We also identified T3Es that differentially regulate flg22-induced reactive oxygen species production and gene expression. Interestingly, several of the NLS-containing T3Es translationally fused with yellow fluorescent protein accumulated in subcellular compartments other than the cell nucleus. Our findings bring new clues to decipher T3E function .
（）是包括韩国在内的全球农作物青枯病的病原体。其毒力主要依赖于III型分泌效应子（T3E）。然而，只有少数T3E得到了表征。在本研究中，我们调查了8个预测含有核定位信号（NLS）的T3E的亚细胞定位以及对植物免疫的操控。虽然其中2个T3E在烟草和本氏烟草中均引发了细胞死亡，但只有1个依赖于Skp1的G2等位基因的抑制子（SGT1），SGT1是核苷酸结合和富含亮氨酸重复免疫受体的分子伴侣。我们还鉴定了差异调节flg22诱导的活性氧产生和基因表达的T3E。有趣的是，几个与黄色荧光蛋白翻译融合的含NLS的T3E积聚在细胞核以外的亚细胞区室中。我们的发现为解读T3E功能带来了新线索。
