Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China.
Theranostics. 2020 Jan 16;10(5):2374-2384. doi: 10.7150/thno.40677. eCollection 2020.
Liquid biopsy has emerged in the last ten years as an appealing noninvasive strategy to support early cancer diagnosis and follow-up interventions. However, conventional liquid biopsy strategies involving specified biomarkers have encountered unexpected inconsistencies stemming from the use of different analytical methodologies. Recent reports have repeatedly demonstrated that integrated detection of multiple liquid biopsy biomarkers can significantly improve diagnostic performance by eliminating the influence of intratumoral heterogeneity. Herein, we review the progress in the field of liquid biopsy and propose a novel integrated liquid biopsy framework consisting of three categories: elementary, intermediate, and advanced integration. We also summarize the merits of the integration strategy and propose a roadmap toward refining cancer diagnosis, metastasis surveillance, and prognostication.
在过去的十年中，液体活检作为一种有吸引力的非侵入性策略，已经出现，以支持早期癌症诊断和后续干预。然而，涉及特定生物标志物的传统液体活检策略由于使用不同的分析方法学而遇到了意想不到的不一致性。最近的报告反复表明，通过消除肿瘤内异质性的影响，对多种液体活检生物标志物进行综合检测可以显著提高诊断性能。在此，我们综述了液体活检领域的进展，并提出了一个由三个部分组成的新型综合液体活检框架：基础整合、中级整合和高级整合。我们还总结了整合策略的优点，并提出了一条通向完善癌症诊断、转移监测和预后的路线图。
