Department of Medical Science, Chungnam National University, Daejeon 35015, Republic of Korea.
Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
Oxid Med Cell Longev. 2020 Jan 31;2020:2946820. doi: 10.1155/2020/2946820. eCollection 2020.
Reactive nitrogen species (RNS), including nitric oxide (NO) has been known as one of the key regulatory molecules in the immune system. In this study, we generated RNS-containing water treated with microwave plasma-generated gas in which the major component was nitric oxide (PGNO), and the effect on the macrophage polarization was investigated. The RNS-containing water was diluted in complete cell culture media (PGNO-solution) into the concentration that did not induce cell death in RAW 264.7 murine macrophages. PGNO-solution upregulates M1-type macrophage activation and downregulates the characteristics of M2-type macrophage at the transcriptional level. In addition, the PGNO-solution-treated M2-like macrophages had higher potential in killing melanoma cells. The anticancer potential was also investigated in a syngeneic mouse model. Our results show that PGNO-solution has the potential to convert the fate of macrophages, suggesting PGNO-solution treatment as a supportive method for controlling the function of macrophages under the tumor microenvironment.
活性氮物种（RNS），包括一氧化氮（NO），已被认为是免疫系统中关键的调节分子之一。在这项研究中，我们生成了含有微波等离子体产生的气体的含 RNS 水，其中主要成分为一氧化氮（PGNO），并研究了其对巨噬细胞极化的影响。将含 RNS 的水用完全细胞培养基（PGNO 溶液）稀释至不会诱导 RAW 264.7 鼠巨噬细胞死亡的浓度。PGNO 溶液在转录水平上上调 M1 型巨噬细胞的激活，并下调 M2 型巨噬细胞的特征。此外，用 PGNO 溶液处理的 M2 样巨噬细胞具有更高的杀伤黑色素瘤细胞的潜力。我们还在同基因小鼠模型中研究了抗癌潜力。我们的结果表明，PGNO 溶液具有改变巨噬细胞命运的潜力，提示 PGNO 溶液处理可能是控制肿瘤微环境中巨噬细胞功能的一种辅助方法。
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