Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, Korea.
Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, Korea.
Sci Rep. 2019 Jan 30;9(1):1011. doi: 10.1038/s41598-018-37711-3.
In this study, we generated water and phosphate buffer treated with microwave plasma-generated gas in which the major component was nitric oxide (PGNO), and investigated the efficiency of the treated water and buffer in fertilization and sanitation. Real time NO level monitored by an electrode sensor was linearly increased over PGNO injection time, and removal of O from liquid before PGNO injection accelerated NO assimilation into liquids. Residual NO was still present 16 h after PGNO injection was stopped. HO, NO, and NO were also detected in PGNO-treated liquids. Spinach plants applied with 10 and 30 times diluted PGNO-treated water and 0.5 mM phosphate buffer showed slightly higher height and dry weight than control after 5 weeks. Plants grown with 10 and 30 times diluted PGNO-treated water exhibited the increased tolerance to water deficiency. Significant anti-microbial activity within 1 h was observed in un-diluted and in half-diluted PGNO-treated water and 0.5 mM phosphate buffer. Our results suggest that water or phosphate buffer containing NO, HO, NO, and NO can be produced by PGNO treatment, and that PGNO-treated water or buffer can be used as a potential fertilizer enhancing plant vitality with sanitation effect.
在这项研究中，我们生成了经微波等离子体产生的气体处理的水和磷酸盐缓冲液，其中主要成分为一氧化氮（PGNO），并研究了处理水和缓冲液在受精和卫生方面的效率。通过电极传感器实时监测到的 NO 水平随 PGNO 注入时间呈线性增加，并且在注入 PGNO 之前从液体中去除 O 会加速 NO 被吸收到液体中。在停止注入 PGNO 16 小时后，仍存在残留的 NO。在 PGNO 处理过的液体中还检测到了 HO、NO 和 NO。菠菜植物应用 10 倍和 30 倍稀释的 PGNO 处理过的水和 0.5mM 磷酸盐缓冲液 5 周后，与对照相比，其高度和干重略有增加。用 10 倍和 30 倍稀释的 PGNO 处理过的水培养的植物表现出对水分缺乏的耐受性增加。在未经稀释和稀释一半的 PGNO 处理过的水中以及 0.5mM 磷酸盐缓冲液中，在 1 小时内观察到显著的抗菌活性。我们的结果表明，水或磷酸盐缓冲液可以通过 PGNO 处理生成含有 NO、HO、NO 和 NO 的物质，并且 PGNO 处理过的水或缓冲液可以用作潜在的肥料，增强植物活力并具有卫生效果。
