Yepes-Molina Lucía, Martínez-Ballesta Maria Carmen, Carvajal Micaela
Plant Nutrition Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, E-30100 Murcia, Spain.
J Adv Res. 2020 Feb 8;23:101-111. doi: 10.1016/j.jare.2020.02.004. eCollection 2020 May.
During the last few years, membrane vesicles (as exovesicles) have emerged as potential nanocarriers for therapeutic applications. They are receiving attention due to their proteo-lipid nature, size, biocompatibility and biodegradability. In this work, we investigated the potential use of isolated root plasma membrane vesicles from broccoli plants as nanocarriers. For that, the entrapment efficiency and integrity of the vesicles were determined. Also, the delivery of keratinocytes and penetrability through skin were studied. The results show that the broccoli vesicles had high stability, in relation to their proteins, and high entrapment efficiency. Also, the interaction between the vesicles and keratinocytes was proven by the delivery of an encapsulated fluorescent product into cells and by the detection of plant proteins in the keratinocyte plasma membrane, showing the interactions between the membranes of two species of distinct biological kingdoms. Therefore, these results, together with the capacity of brassica vesicles to cross the skin layers, detected by fluorescent penetration, enable us to propose a type of nanocarrier obtained from natural plant membranes for use in transdermal delivery.
在过去几年中，膜囊泡（作为外泌体）已成为治疗应用中潜在的纳米载体。由于其蛋白-脂质性质、大小、生物相容性和生物降解性，它们受到了关注。在这项工作中，我们研究了从西兰花植株中分离出的根质膜囊泡作为纳米载体的潜在用途。为此，测定了囊泡的包封效率和完整性。此外，还研究了其对角质形成细胞的递送以及透过皮肤的穿透性。结果表明，西兰花囊泡相对于其蛋白质具有高稳定性和高包封效率。此外，通过将封装的荧光产物递送至细胞以及检测角质形成细胞质膜中的植物蛋白，证明了囊泡与角质形成细胞之间的相互作用，显示了两个不同生物界的膜之间的相互作用。因此，这些结果，连同通过荧光穿透检测到的芸苔属囊泡穿过皮肤层的能力，使我们能够提出一种从天然植物膜获得的用于透皮递送的纳米载体类型。
