Department of Applied Bioresource Science, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea.
Department of Ecology and Conservation, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea.
Biomolecules. 2020 Feb 19;10(2):333. doi: 10.3390/biom10020333.
Lectins have the ability to bind specific carbohydrates and they have potential applications as medical and pharmacological agents. The unique structure and usefulness of red algal lectin have been reported, but these lectins are limited to a few marine algal groups. In this study, a novel mannose-binding lectin from ( lectin, GCL) was purified using antiviral screens and affinity chromatography. We characterized the molecular weight, agglutination activity, hemagglutination activity, and heat stability of GCL. To determine the carbohydrate specificity, a glycan microarray was performed. GCL showed strong binding affinity for Maltohexaose-β-Sp1 and Maltoheptaose-β-Sp1 with weak affinity for other monosaccharides and preferred binding to high-mannan structures. The N-terminal sequence and peptide sequence of GCL were determined using an Edman degradation method and LC-MS/MS, and the cDNA and peptide sequences were deduced. GCL was shown to consist of 231 amino acids (24.9 kDa) and the N-terminus methionine was eliminated after translation. GCL possessed a tandem repeat structure of six domains, similar to the other red algal lectins. The mannose binding properties and tandem repeat structure of GCL may confer it the potential to act as an antiviral agent for protection against viral infection.
凝集素具有结合特定碳水化合物的能力，它们有可能作为医学和药理学制剂得到应用。已报道了红藻凝集素的独特结构和用途，但这些凝集素仅限于少数海洋藻类群体。在这项研究中，我们使用抗病毒筛选和亲和层析从 （凝集素，GCL）中纯化了一种新型的甘露糖结合凝集素。我们对 GCL 的分子量、凝集活性、血凝活性和热稳定性进行了表征。为了确定糖的特异性，我们进行了聚糖微阵列分析。GCL 对 Maltohexaose-β-Sp1 和 Maltoheptaose-β-Sp1 具有很强的结合亲和力，对其他单糖的亲和力较弱，并且优先与高甘露聚糖结构结合。我们使用 Edman 降解法和 LC-MS/MS 确定了 GCL 的 N 末端序列和肽序列，并推导出了 cDNA 和肽序列。GCL 由 231 个氨基酸（24.9 kDa）组成，翻译后 N 末端的蛋氨酸被切除。GCL 具有类似于其他红藻凝集素的六个结构域的串联重复结构。GCL 的甘露糖结合特性和串联重复结构可能使其具有作为抗病毒剂的潜力，以防止病毒感染。
