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土耳其一家三级医院分离菌株的抗菌药物耐药性与分子流行病学

Antimicrobial Resistance and Molecular Epidemiology of Isolated in a Tertiary Hospital in Turkey.

作者信息

Asgin Nergis, Otlu Baris

机构信息

Department of Medical Microbiology, Faculty of Medicine, Karabuk University,78100 Karabuk, Turkey.

Department of Medical Microbiology, Faculty of Medicine, Inonu University,44280 Malatya, Turkey.

出版信息

Pathogens. 2020 Feb 19;9(2):136. doi: 10.3390/pathogens9020136.

Abstract

Although is part of the human flora, it has recently drawn attention both for its multidrug resistance and its role as an invasive infection/outbreak agent. This cross-sectional study aimed to determine the antimicrobial resistance and clonal relationships among strains. In total, 81 strains were identified using Phoenix-100 (BD, Sparks, MD, USA). The antimicrobial resistance of the strains was determined using the Kirby-Bauer disk diffusion method. Clonal relatedness among the strains was performed via arbitrarily primed polymerase chain reaction (AP-PCR). All 81 strains were resistant to penicillin, cefotaxime, ciprofloxacin, and tetracycline, but susceptible to vancomycin and linezolid. The resistance rates to gentamicin, erythromycin, and clindamycin were 34.6%, 79%, and 87.7% respectively. AP-PCR results showed no predominant clone among the strains. is reportedly the cause of an increasing number of invasive infections/outbreaks. Moreover, treatment options are limited. The study showed that vancomycin, linezolid, and gentamicin can be selected for the empirical treatment of infections. Although no single-clone outbreak was observed in our hospital, small clonal circulations were observed within some units, indicating cross-contamination. Therefore, a comprehensive infection control program is warranted in future.

摘要

虽然[具体菌种名称]是人体菌群的一部分,但它最近因其多重耐药性以及作为侵袭性感染/暴发病原体的作用而受到关注。这项横断面研究旨在确定[具体菌种名称]菌株之间的抗菌药物耐药性和克隆关系。总共使用Phoenix-100(美国马里兰州斯帕克斯市BD公司)鉴定出81株[具体菌种名称]菌株。采用 Kirby-Bauer 纸片扩散法测定菌株的抗菌药物耐药性。通过任意引物聚合酶链反应(AP-PCR)分析菌株之间的克隆相关性。所有81株[具体菌种名称]菌株均对青霉素、头孢噻肟、环丙沙星和四环素耐药,但对万古霉素和利奈唑胺敏感。对庆大霉素、红霉素和克林霉素的耐药率分别为34.6%、79%和87.7%。AP-PCR结果显示,[具体菌种名称]菌株中没有优势克隆。据报道,[具体菌种名称]是侵袭性感染/暴发数量增加的原因。此外,治疗选择有限。该研究表明,万古霉素、利奈唑胺和庆大霉素可用于[具体菌种名称]感染的经验性治疗。虽然在我们医院未观察到单克隆暴发,但在一些科室中观察到了小范围的克隆传播,这表明存在交叉污染。因此,未来有必要制定全面的感染控制计划。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e23/7168331/c2ac21247dfa/pathogens-09-00136-g001.jpg

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