Asgin Nergis, Otlu Baris
Department of Medical Microbiology, Faculty of Medicine, Karabuk University,78100 Karabuk, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Inonu University,44280 Malatya, Turkey.
Pathogens. 2020 Feb 19;9(2):136. doi: 10.3390/pathogens9020136.
Although is part of the human flora, it has recently drawn attention both for its multidrug resistance and its role as an invasive infection/outbreak agent. This cross-sectional study aimed to determine the antimicrobial resistance and clonal relationships among strains. In total, 81 strains were identified using Phoenix-100 (BD, Sparks, MD, USA). The antimicrobial resistance of the strains was determined using the Kirby-Bauer disk diffusion method. Clonal relatedness among the strains was performed via arbitrarily primed polymerase chain reaction (AP-PCR). All 81 strains were resistant to penicillin, cefotaxime, ciprofloxacin, and tetracycline, but susceptible to vancomycin and linezolid. The resistance rates to gentamicin, erythromycin, and clindamycin were 34.6%, 79%, and 87.7% respectively. AP-PCR results showed no predominant clone among the strains. is reportedly the cause of an increasing number of invasive infections/outbreaks. Moreover, treatment options are limited. The study showed that vancomycin, linezolid, and gentamicin can be selected for the empirical treatment of infections. Although no single-clone outbreak was observed in our hospital, small clonal circulations were observed within some units, indicating cross-contamination. Therefore, a comprehensive infection control program is warranted in future.
虽然[具体菌种名称]是人体菌群的一部分,但它最近因其多重耐药性以及作为侵袭性感染/暴发病原体的作用而受到关注。这项横断面研究旨在确定[具体菌种名称]菌株之间的抗菌药物耐药性和克隆关系。总共使用Phoenix-100(美国马里兰州斯帕克斯市BD公司)鉴定出81株[具体菌种名称]菌株。采用 Kirby-Bauer 纸片扩散法测定菌株的抗菌药物耐药性。通过任意引物聚合酶链反应(AP-PCR)分析菌株之间的克隆相关性。所有81株[具体菌种名称]菌株均对青霉素、头孢噻肟、环丙沙星和四环素耐药,但对万古霉素和利奈唑胺敏感。对庆大霉素、红霉素和克林霉素的耐药率分别为34.6%、79%和87.7%。AP-PCR结果显示,[具体菌种名称]菌株中没有优势克隆。据报道,[具体菌种名称]是侵袭性感染/暴发数量增加的原因。此外,治疗选择有限。该研究表明,万古霉素、利奈唑胺和庆大霉素可用于[具体菌种名称]感染的经验性治疗。虽然在我们医院未观察到单克隆暴发,但在一些科室中观察到了小范围的克隆传播,这表明存在交叉污染。因此,未来有必要制定全面的感染控制计划。