Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, China.
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Antonie Van Leeuwenhoek. 2023 Sep;116(9):907-918. doi: 10.1007/s10482-023-01855-8. Epub 2023 Jun 27.
Corynebacterium striatum is an emerging, multidrug-resistant pathogen that frequently causes nosocomial infections worldwide. This study aimed to investigate phylogenetic relationship and presence of genes responsible for antimicrobial resistance among C. striatum strains associated with an outbreak at the Shanxi Bethune Hospital, China, in 2021. Fecal samples were collected from 65 patients with C. striatum infection at Shanxi Bethune Hospital between February 12, 2021 and April 12, 2021. C. striatum isolates were identified by 16S rRNA and rpoB gene sequencing. E-test strips were used to examine the antimicrobial susceptibility of the isolates. Whole-genome sequencing and bioinformatics analysis were employed to assess the genomic features and identify antimicrobial resistance genes of the isolates. Crystal violet staining was conducted to determine the ability of biofilm formation of each isolate. A total of 64 C. striatum isolates were identified and categorized into 4 clades based on single nucleotide polymorphisms. All isolates were resistant to penicillin, meropenem, ceftriaxone, and ciprofloxacin but susceptible to vancomycin and linezolid. Most isolates were also resistant to tetracycline, clindamycin, and erythromycin, with susceptibility rates of 10.77, 4.62, and 7.69%, respectively. Genomic analysis revealed 14 antimicrobial resistance genes in the isolates, including tetW, ermX, and sul1. Crystal violet staining showed that all isolates formed biofilms on the abiotic surface. Four clades of multidrug-resistant C. striatum spread in our hospitals possibly due to the acquisition of antimicrobial resistance genes.
聚集棒状杆菌是一种新兴的、多药耐药病原体，它经常在全球范围内引起医院感染。本研究旨在调查与中国山西白求恩医院 2021 年暴发疫情相关的聚集棒状杆菌菌株的系统发育关系和与抗菌药物耐药相关的基因。于 2021 年 2 月 12 日至 2021 年 4 月 12 日，从山西白求恩医院 65 例聚集棒状杆菌感染患者的粪便样本中采集了粪便样本。通过 16S rRNA 和 rpoB 基因测序鉴定聚集棒状杆菌分离株。采用 E 试验条检测分离株的抗菌药物敏感性。采用全基因组测序和生物信息学分析评估分离株的基因组特征和鉴定抗菌药物耐药基因。采用结晶紫染色法测定各分离株的生物膜形成能力。共鉴定出 64 株聚集棒状杆菌，并根据单核苷酸多态性将其分为 4 个分支。所有分离株均对青霉素、美罗培南、头孢曲松和环丙沙星耐药，但对万古霉素和利奈唑胺敏感。大多数分离株还对四环素、克林霉素和红霉素耐药，敏感性分别为 10.77%、4.62%和 7.69%。基因组分析显示，分离株中存在 14 种抗菌药物耐药基因，包括 tetW、ermX 和 sul1。结晶紫染色显示，所有分离株均在非生物表面形成生物膜。四个分支的多药耐药聚集棒状杆菌可能由于获得了抗菌药物耐药基因而在我们的医院中传播。
