Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Sci Rep. 2020 Feb 24;10(1):3318. doi: 10.1038/s41598-020-60150-y.
Reducing dietary crude protein (CP) beyond a certain threshold leads to poor growth performance in pigs; however, the underlying mechanisms are not well understood. Following an adaption period, thirty-seven weaned pigs were weight matched (8.41 ± 0.14 kg), housed individually and randomly assigned into three groups with different dietary CP levels: 24% CP (CON; n = 12), 18% CP (n = 12) and 12% CP (n = 13) for 28 days. The body weight was not different between the CON and 18% CP diets, but 12% CP significantly decreased body weight after day 21. Compared to the CON, pigs fed with 12% CP decreased feed intake day 17 onwards. The 12% CP diet increased the energy expenditure during week 1 compared to the CON. The 12% CP influenced starch and sucrose, nitrogen, and branched-chain amino acids metabolism pathways. The feces of pigs fed with 12% CP were less enriched in Prevotella, but had higher relative abundance of Christensenedilaceae, Aligiphilus and Algoriphagus than CON and 18% CP. Overall, reducing dietary CP by 50%, but not by 25%, significantly influenced the physiological responses in nursery pigs. The pigs fed with low or standard protein diets had differential bacterial communities in their feces as well as serum metabolomics profile.
在一定阈值以下降低饲粮粗蛋白（CP）会导致猪生长性能下降；然而，其潜在机制尚不清楚。在适应期后，将 37 头断奶猪按体重匹配（8.41±0.14kg），单独饲养并随机分为三组，分别饲喂不同 CP 水平的饲粮：24% CP（CON；n=12）、18% CP（n=12）和 12% CP（n=13），试验期 28 天。CON 和 18% CP 饲粮之间的体重没有差异，但 12% CP 组在第 21 天体重显著下降。与 CON 相比，饲喂 12% CP 的猪从第 17 天开始采食量下降。与 CON 相比，12% CP 饲粮在第 1 周增加了能量消耗。12% CP 影响了淀粉和蔗糖、氮和支链氨基酸代谢途径。饲喂 12% CP 的猪粪便中普雷沃氏菌减少，但 Christensenedilaceae、Aligiphilus 和 Algoriphagus 的相对丰度高于 CON 和 18% CP。总之，饲粮 CP 降低 50%（而非 25%）显著影响了保育猪的生理反应。饲喂低蛋白或标准蛋白饲粮的猪粪便中细菌群落以及血清代谢组学图谱存在差异。
