Onoja A B, Maina M M, Opayele A V
Department of Virology, College of Medicine, University of Ibadan, Nigeria.
Department of Veterinary Microbiology, University of Maiduguri, Nigeria.
Niger Vet J. 2018;39(4):285-294. Epub 2018 Dec 13.
West Nile virus (WNV) is maintained in an enzootic cycle between ornithophilic mosquitoes and birds. There is paucity of information on population density of these vectors and the molecular details in Nigeria. We determined relative abundance and employed molecular methods to detect . Mosquitoes were caught from April 2013 to February 2014. Genomic DNA extraction was carried out and acetylcholinesterase 2 gene amplified by Polymerase Chain Reaction in different . and were identified in different locations, while some other could not be identified. Culex p. quinquefasciatus were sequenced by Sanger's method. Vector density was 100 per man hour. Identification of from different locations in Ibadan, Nigeria reaffirms the presence of vectors and highlights possibility of enzootic transmission of WNV. Surveillance should be sustained in equine population and bird reservoirs.
西尼罗河病毒（WNV）在嗜鸟蚊子和鸟类之间维持着一种地方性循环。在尼日利亚，关于这些病媒的种群密度以及分子细节的信息匮乏。我们测定了相对丰度，并采用分子方法进行检测。于2013年4月至2014年2月捕获蚊子。进行了基因组DNA提取，并通过聚合酶链反应在不同……中扩增乙酰胆碱酯酶2基因。在不同地点鉴定出了……和……，而其他一些……无法鉴定。用桑格法对致倦库蚊进行了测序。病媒密度为每人工小时100只。对来自尼日利亚伊巴丹不同地点……的鉴定再次证实了病媒的存在，并突出了西尼罗河病毒地方性传播的可能性。应对马群和鸟类宿主持续进行监测。
