Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia.
Laboratoire de Microbiologie Alimentaire, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, University of Carthage, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia.
Acta Parasitol. 2022 Mar;67(1):351-355. doi: 10.1007/s11686-021-00467-4. Epub 2021 Sep 8.
Food-borne Toxoplasma gondii infection is ranked among parasitic diseases of global concern. Toxoplasmosis is qualified as a one health disease, since it affects human health, animals, and ecosystems. The present study was performed to estimate the molecular prevalence of T. gondii in industrial poultry meat samples from Tunisia. A total of 140 breast samples were collected from chicken (Gallus gallus domesticus) and turkey (Meleagris gallopavo) and four breeding systems (broiler chickens, reform laying hens, chicken farm label, and broiler turkeys) cover the majority of chicken meat production in Tunisia.
For each sample, DNA was extracted and T. gondii-specific PCR, targeting the B1 gene was performed.
The overall molecular prevalence of T. gondii in poultry meat samples was 20% (28/140; 95% CI: 13.3-26.6%). The highest prevalence was observed in chicken farm label (25.7%; 9/35). Prevalence rates were 20.0% (7/35), 20.0% (7/35), and 14.3% (5/35) in broiler chickens, broiler turkeys, and reform laying hens, respectively (p > 0.05).
Our finding suggests the involvement of industrial chicken meat, as a potential risk factor of human T. gondii transmission, especially when the latter is consumed undercooked.
食源性刚地弓形虫感染被列为全球关注的寄生虫病之一。弓形虫病被认为是一种涉及人类健康、动物和生态系统的“同一健康”疾病。本研究旨在估计突尼斯工业家禽肉样本中刚地弓形虫的分子流行率。从鸡（Gallus gallus domesticus）和火鸡（Meleagris gallopavo）中采集了 140 个乳房样本，以及四种养殖系统（肉鸡、改造蛋鸡、鸡场标签和肉鸡火鸡），涵盖了突尼斯大部分鸡肉生产。
对每个样本进行 DNA 提取，然后进行针对 B1 基因的刚地弓形虫特异性 PCR。
家禽肉样本中刚地弓形虫的总体分子流行率为 20%（28/140；95%CI：13.3-26.6%）。鸡场标签的流行率最高（25.7%；9/35）。肉鸡、肉鸡火鸡和改造蛋鸡的流行率分别为 20.0%（7/35）、20.0%（7/35）和 14.3%（5/35）（p>0.05）。
我们的发现表明，工业鸡肉可能是人类刚地弓形虫传播的潜在危险因素，尤其是在未煮熟的情况下食用时。
