Patel H V, Morton D J
Department of Pharmacy, University of Zimbabwe, Harare.
J Clin Pharm Ther. 1988 Jun;13(3):233-8. doi: 10.1111/j.1365-2710.1988.tb00186.x.
A simple, economical colorimetric procedure for the determination of Paracetamol in serum or plasma (50-450 micrograms/ml) is described. Using the described method it is possible to quantify Paracetamol concentrations within 25 min which makes the technique ideal for use in an emergency-suspected overdose situation. A total of 178 drug/drug combination products were examined for possible interference with the method and of these, eight were shown to interfere significantly with the quantitative assay. However, in all cases it would be possible to correct for the interference to arrive at a semi-quantitative value for Paracetamol concentration by using the absorbance and by comparing the visible colour of the test sample with the Paracetamol standards.
本文描述了一种简单、经济的比色法，用于测定血清或血浆中对乙酰氨基酚（浓度范围为50 - 450微克/毫升）。使用所述方法，可在25分钟内定量对乙酰氨基酚浓度，这使得该技术非常适合用于疑似过量服药的紧急情况。总共检测了178种药物/药物组合产品对该方法可能的干扰，其中有8种显示出对定量测定有显著干扰。然而，在所有情况下，通过使用吸光度并将测试样品的可见颜色与对乙酰氨基酚标准品进行比较，有可能校正干扰以得出对乙酰氨基酚浓度的半定量值。
