Chambers R E, Jones K
Ann Clin Biochem. 1976 Jul;13(4):433-4. doi: 10.1177/000456327601300132.
Plasma paracetamol levels have generally been determined either by gas chromatography (Stewart and Willis, 1975), which relies on complex equipment, or by spectrophotometry (Knepil, 1974), which can be time-consuming. The introduction by Glynn and Kendal (1975) of a simple colorimetric method based on the reaction of paracetamol with nitrous acid to give 2-nitro-4-acetamidophenol appears to have overcome these disadvantages, thereby providing a suitable procedure for the rapid measurement of plasma paracetamol in cases of overdose. The method was reported to be specific for paracetamol, no interference being caused either by the sulphate and glucuronide conjugates of paracetamol or by a large number of other commonly found drugs. This communication presents the results of a study in which plasma paracetamol levels determined by the colorimetric method were compared with those determined by an established gas chromatographic technique.
血浆中对乙酰氨基酚的含量通常采用气相色谱法（Stewart和Willis，1975年）测定，该方法依赖复杂的设备，或者采用分光光度法（Knepil，1974年）测定，该方法可能耗时较长。Glynn和Kendal（1975年）引入了一种基于对乙酰氨基酚与亚硝酸反应生成2-硝基-4-乙酰氨基酚的简单比色法，似乎克服了这些缺点，从而为过量用药情况下快速测定血浆中对乙酰氨基酚提供了一种合适的方法。据报道，该方法对对乙酰氨基酚具有特异性，对乙酰氨基酚的硫酸盐和葡萄糖醛酸结合物或大量其他常见药物均无干扰。本通讯介绍了一项研究结果，其中将比色法测定的血浆对乙酰氨基酚水平与既定的气相色谱技术测定的水平进行了比较。
