Department of Biological Sciences, College of Superior Studies Cuautitlan, The National Autonomous University of Mexico, Cuautitlan Izcalli, Mexico.
Department of Pathology, College of Veterinary Medicine, The University of Georgia, Athens, Georgia.
Microbiol Immunol. 2020 May;64(5):366-376. doi: 10.1111/1348-0421.12782. Epub 2020 Mar 26.
Worldwide, many emerging porcine parvoviruses (PPVs) have been linked to porcine circovirus-2 (PCV2) associated disease (PCVAD), which includes post-weaning multi-systemic wasting syndrome (PMWS), PCV2-related reproductive failure (PCV2-RF), as well as other syndromes. To determine the DNA prevalence of PPVs and their relationship with PMWS and PCV2-RF in Mexico, 170 formalin-fixed paraffin-embedded tissues were selected from archival collections to detect PPVs using a nested polymerase chain reaction. The tissues were composed of 50 PMWS cases, 20 age-matched tissues from healthy pigs, 56 PCV2-related reproductive failure (PCV2 -RF) cases, and 44 PCV2 -RF cases. Overall, PPV2 and PPV6 were the most prevalent species (90.0% and 74.7%, respectively). In 8-11 week old pigs, the highest prevalence was for PPV6 and PPV3. Concerning reproductive failure, the PCV2-affected farms had a significantly higher prevalence for PPV6 (61.6%) and PPV5 (36.4%) than the PCV2-unaffected farms (35.0% and 5.0%, respectively). The concurrent infection rate was high, being significant for PPV2/PPV4 and PPV1/PPV5 within the PMWS cases and for PPV6/PPV5 among the PCV2 -RF tissues. PPV5 showed a significant relationship with PMWS, whereas PPV5 and PPV6 were significant for PCVAD. The prevalence and coinfection rate of PPVs in Mexico were markedly higher than that described in other countries, denoting that PPV5 and PPV6 might have a potential role in PCVAD in Mexico. It is concluded that it is likely that the density population of pigs in Mexico is contributing to high PPV inter-species and PCV2 coinfections which might lead to a different pathogenic outcome.
在全球范围内，许多新兴的猪细小病毒（PPV）与猪圆环病毒 2 型（PCV2）相关疾病（PCVAD）有关，包括断奶后多系统消耗综合征（PMWS）、PCV2 相关繁殖障碍（PCV2-RF）以及其他综合征。为了确定 PPV 在墨西哥的流行情况及其与 PMWS 和 PCV2-RF 的关系，从档案库中选择了 170 份福尔马林固定石蜡包埋组织，使用巢式聚合酶链反应检测 PPV。这些组织包括 50 个 PMWS 病例、20 个来自健康猪的年龄匹配组织、56 个 PCV2-RF 病例和 44 个 PCV2-RF 病例。总体而言，PPV2 和 PPV6 是最常见的物种（分别为 90.0%和 74.7%）。在 8-11 周龄的猪中，PPV6 和 PPV3 的流行率最高。关于繁殖障碍，受 PCV2 影响的农场中 PPV6（61.6%）和 PPV5（36.4%）的流行率明显高于未受 PCV2 影响的农场（分别为 35.0%和 5.0%）。同时感染率较高，在 PMWS 病例中，PPV2/PPV4 和 PPV1/PPV5 的同时感染率显著，在 PCV2-RF 组织中，PPV6/PPV5 的同时感染率显著。PPV5 与 PMWS 有显著关系，而 PPV5 和 PPV6 与 PCVAD 有显著关系。墨西哥的 PPV 流行率和合并感染率明显高于其他国家，这表明 PPV5 和 PPV6 可能在墨西哥的 PCVAD 中发挥潜在作用。结论是，墨西哥猪的高密度种群可能导致 PPV 种间和 PCV2 合并感染率较高，从而导致不同的发病结果。
