Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Mitochondrion. 2020 May;52:67-74. doi: 10.1016/j.mito.2020.02.009. Epub 2020 Feb 22.
The delivery of nucleic acids targeting mutant mtDNA represent a potential strategy for addressing a variety of mitochondria-related diseases. We previously developed a MITO-Porter, a nano carrier that is capable of delivering nanoparticles of nucleic acids to mitochondria of human cells. Here, we report on an investigation of a series of nanoparticles formed with various poly cationic peptides that can release nucleic acids in response to a mitochondrial environment. A significant relationship was found between the number of and the location of arginine and histidine residues in the peptide sequence and the release of nucleic acids in a mitochondrial environment.
靶向突变 mtDNA 的核酸递呈代表了一种解决各种与线粒体相关疾病的潜在策略。我们之前开发了一种 MITO-Porter，这是一种纳米载体，能够将核酸的纳米颗粒递送至人细胞的线粒体中。在这里，我们报告了一系列由各种阳离子肽形成的纳米颗粒的研究，这些肽可以在响应线粒体环境时释放核酸。在肽序列中精氨酸和组氨酸残基的数量和位置与在线粒体环境中释放核酸之间存在显著关系。
