Kubena K S, Edgar S E, Veltmann J R
Department of Animal Science (Human Nutrition), Texas A&M University, College Station 77843.
J Am Coll Nutr. 1988 Aug;7(4):317-24. doi: 10.1080/07315724.1988.10720249.
Eight-eight female weanling Sprague-Dawley rats were fed diets containing either 650 or 150 mg magnesium/kg diet and 7.0 or 3.5 mg pyridoxine-HCl/kg diet, in a 2 x 2 factorial arrangement, during growth, gestation, and lactation. The objective of the study was to determine whether concurrent dietary deficiencies of magnesium and pyridoxine were synergistic, additive, or antagonistic with regards to effects on reproductive performance, growth, and development of offspring, and tissue content of magnesium and calcium. Body weight of dams and pups was not different between groups until day 9 of lactation, at which point those animals in either low magnesium group weighed less than the other. Litter size and birth weight were not different. Development, as measured by timing of unfolding of the external ear, opening of both eyes, and clinical emergence of incisors, was delayed in pups from litters in the low magnesium groups. A synergistic effect on delay of onset of ear unfolding by deficiency of both magnesium and pyridoxine was observed. Calcium content of heart and kidney from dams was increased in the low magnesium groups. Renal calcium was not further increased by the level of pyridoxine deficiency in this study. The calcium to magnesium ratio in heart from pups was higher in those from litters in the low magnesium and pyridoxine group than in the others. Results indicate that simultaneous deficiencies of magnesium and pyridoxine may impair function synergistically. Because these two nutrients are often reported to be presented in inadequate amounts in diets of women in their reproductive years, the potential exists for impaired reproductive success.
88只雌性断乳斯普拉格-道利大鼠在生长、妊娠和哺乳期被给予含有650或150毫克镁/千克日粮以及7.0或3.5毫克盐酸吡哆醇/千克日粮的饲料，采用2×2析因设计。本研究的目的是确定镁和吡哆醇同时缺乏在对生殖性能、生长、后代发育以及镁和钙的组织含量的影响方面是协同、相加还是拮抗的。直到哺乳期第9天，各组间母鼠和幼鼠的体重没有差异，此时低镁组的动物体重低于其他组。窝仔数和出生体重没有差异。低镁组窝仔的幼鼠在通过外耳展开时间、双眼睁开时间和门齿临床萌出时间来衡量的发育方面有所延迟。观察到镁和吡哆醇同时缺乏对耳展开起始延迟有协同作用。低镁组母鼠心脏和肾脏的钙含量增加。在本研究中，肾脏钙含量并未因吡哆醇缺乏水平而进一步增加。低镁和吡哆醇组窝仔的幼鼠心脏中的钙镁比高于其他组。结果表明，镁和吡哆醇同时缺乏可能协同损害功能。由于经常报道这两种营养素在育龄妇女的饮食中含量不足，存在生殖成功率受损的可能性。
