Felice J H, Kirksey A
J Nutr. 1981 Apr;111(4):610-7. doi: 10.1093/jn/111.4.610.
Effects of a dietary deficiency of vitamin B-6 imposed during the lactation period on the level of this vitamin in milk, liver and muscle were assessed in rats. A control diet (vitamin B-6 free basal diet supplemented with 10 mg pyridoxine . HCl/kg) was fed to all rats just before and during the gestation period. On day 1 of lactation, dams were randomly assigned to three dietary treatment groups: deficient (vitamin B-6 free basal diet), control or restricted control (control diet restricted in quantity to that consumed by the deficient group). Levels of total vitamin B-6 in milk, liver and muscle were determined by microbiological assay on days 1, 6, 16 and 21 of lactation. On day 6, the concentration of vitamin B-6 in milk of deficient dams was less than 20% of control or restricted control values. The low level of vitamin B-6 in milk of the deficient group continued to be evident on days 16 and 21 whereas in control and restricted control groups the vitamin content increased on days 16 and 21 compared to the values on day 6. In contrast to the low level of vitamin B-6 content observed in milk on day 6 in the deficient group, the vitamin content in liver of this group was approximately 75% of control or restricted control values, and the vitamin content in muscle was not significantly altered by the deficiency. Hence, milk was effected sooner by the deficiency and was an earlier indicator of impending vitamin B-6 deficiency than was liver or muscle tissues.
研究人员评估了哺乳期维生素B-6膳食缺乏对大鼠乳汁、肝脏和肌肉中该维生素水平的影响。在妊娠前及妊娠期，所有大鼠均喂食对照饮食（不含维生素B-6的基础饮食，补充10毫克盐酸吡哆醇/千克）。在哺乳期第1天，将母鼠随机分为三个膳食处理组：缺乏组（不含维生素B-6的基础饮食）、对照组或限制对照组（对照饮食，量限制为缺乏组的摄入量）。在哺乳期第1、6、16和21天，通过微生物学测定法测定乳汁、肝脏和肌肉中总维生素B-6的水平。在第6天，缺乏组母鼠乳汁中维生素B-6的浓度低于对照组或限制对照组的20%。缺乏组乳汁中维生素B-6的低水平在第16天和第21天仍然明显，而对照组和限制对照组中，维生素含量在第16天和第21天相比第6天有所增加。与缺乏组第6天乳汁中观察到的低维生素B-6含量相反，该组肝脏中的维生素含量约为对照组或限制对照组的75%，且缺乏对肌肉中的维生素含量没有显著影响。因此，乳汁比肝脏或肌肉组织更早受到缺乏的影响，是即将出现维生素B-6缺乏的早期指标。
