Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914, Rasht, Iran.
Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914, Rasht, Iran.
Bioelectrochemistry. 2020 Jun;133:107489. doi: 10.1016/j.bioelechem.2020.107489. Epub 2020 Feb 19.
A label-free electrochemical progesterone (P4) aptasensor was successfully developed by covalently immobilizing NH-functionalized P4-specific aptamer on the electrode surface. The NiO-Au hybrid nanofibers were synthesized by the electrospinning technique. GQDs-NiO-AuNFs nanocomposite was prepared by dispersing of electrospun NiO-AuNFs in the as-synthesized graphene quantum dots (GQDs) solution and stirring for 24 h. Novel GQDs-NiO-AuNFs nano-architecture in combination with functionalized multiwalled carbon nanotubes (f-MWCNTs) were further utilized to modify screen printed carbon electrode (SPCE) in order to construct an effective immobilization matrix with plenty of carboxylic functional groups. The stepwise assembly process of the designed aptasensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The aptamer-progesterone complex formation led to a hindered electron transfer reaction on the sensing interface, which decreased the redox probe peak current. Based on of this, progesterone could be quantitatively detected by monitoring the decrease of differential pulse voltammetric (DPV) responses of [Fe(CN)] peak current with increasing the progesterone concentration. Under optimized experimental parameters, the aptasensor exhibited a dynamic concentration range from 0.01 to 1000 nM and a detection limit of 1.86 pM. The proposed aptasensor was successfully employed for the determination of progesterone in human serum samples and pharmaceutical formulations.
一种无标记电化学孕酮（P4）适体传感器通过将 NH 功能化的 P4 特异性适体共价固定在电极表面上成功开发。NiO-Au 杂化纳米纤维通过静电纺丝技术合成。通过将电纺 NiO-AuNFs 在合成的石墨烯量子点（GQDs）溶液中分散并搅拌 24 小时制备 GQDs-NiO-AuNFs 纳米复合材料。新型 GQDs-NiO-AuNFs 纳米结构与功能化多壁碳纳米管（f-MWCNTs）进一步用于修饰丝网印刷碳电极（SPCE），以构建具有丰富羧基官能团的有效固定基质。设计的适体传感器的分步组装过程通过循环伏安法（CV）和电化学阻抗谱（EIS）进行了表征。适体-孕酮复合物的形成导致传感界面上的电子转移反应受阻，从而降低了氧化还原探针峰电流。基于此，可以通过监测[Fe（CN）]峰电流的差分脉冲伏安法（DPV）响应随孕酮浓度增加而降低来定量检测孕酮。在优化的实验参数下，该适体传感器表现出从 0.01 到 1000 nM 的动态浓度范围和 1.86 pM 的检测限。该适体传感器成功用于人血清样品和药物制剂中孕酮的测定。
