Lopes Graciliana, Clarinha Duarte, Vasconcelos Vitor
CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
FCUP, Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
Microorganisms. 2020 Feb 21;8(2):302. doi: 10.3390/microorganisms8020302.
In this study, five cyanobacteria strains ( LEGE15481, LEGE12431, () LEGE13457, LEGE03282 and -like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193-63926 μg/g dry extract), with all--carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC values (0.29-0.38 mg dry extract/mL) regarding O scavenging, and revealed anti-inflammatory potential, with LEGE13457, LEGE15481 and -like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% ( < 0.05). With the exception of LEGE15481, all the extracts significantly reduced keratinocyte proliferation ( < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, -like sp. LEGE13412 and LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential.
在本研究中，对来自CIIMAR的蓝色生物技术与生态毒理学培养物保藏中心（LEGE CC）的五株蓝藻菌株（LEGE15481、LEGE12431、（）LEGE13457、LEGE03282和类LEGE13412菌株）在治疗银屑病方面的生物技术潜力进行了探索。通过高效液相色谱-光电二极管阵列检测（HPLC-PDA）对不同提取物的色素谱进行了表征。评估了提取物对超氧阴离子自由基（O）的抗氧化潜力。分别使用巨噬细胞RAW 264.7和人角质形成细胞HaCaT作为细胞系模型，在体外评估了它们的抗炎和抗增殖潜力。陆生和淡水菌株的类胡萝卜素含量最高（33193 - 63926μg/g干提取物），其中全反式β-胡萝卜素、玉米黄质、海胆酮和叶黄素衍生物是最丰富的类胡萝卜素。丙酮是色素提取最有效的溶剂。丙酮提取物在清除O方面的IC值最低（0.29 - 0.38mg干提取物/mL），并显示出抗炎潜力，LEGE13457、LEGE15481和类LEGE13412菌株使RAW 264.7细胞培养基中的一氧化氮（NO）减少了约25%（P < 0.05）。除LEGE15481外，所有提取物均显著降低了角质形成细胞的增殖（P < 0.05），表明在不同细胞系之间存在选择性毒性。总体而言，类LEGE13412菌株和LEGE13457菌株因其抗氧化、抗炎和抗增殖潜力，在银屑病治疗框架内似乎具有进一步开发的前景。
