大鼠肾热缺血及白藜芦醇保护作用的组织形态计量学评价。
Histomorphometric evaluation of the rat kidney submitted to warm ischemia and the protective effect of resveratrol.
机构信息
Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Veterinary Medicine, Educational Foundation Serra Dos Órgãos, Teresópolis, Brazil.
出版信息
Am J Surg. 2020 Oct;220(4):1119-1123. doi: 10.1016/j.amjsurg.2020.02.025. Epub 2020 Feb 19.
BACKGROUND
The objective of this study is to quantitatively evaluate the protective effects of resveratrol for using during renal warm ischemia.
METHODS
Rats were allocated into 4 groups: Sham, Sham Resveratrol, Ischemia, Ischemia Resveratrol. Sham Resveratrol and Ischemia Resveratrol received resveratrol before surgery. Ischemia and Ischemia Resveratrol had renal vessels clamped. Animals were euthanized four weeks after. Serum urea and creatinine were measured. Renal weight and volume, cortex-non-cortex areas ratio, cortical volume, glomerular volumetric density, volume-weighted mean glomerular volume and number of glomeruli per kidney were evaluated.
RESULTS
Serum urea in Ischemia increased by 10.4% compared to Sham and no differences were observed among Ischemia Resveratrol and sham groups. The glomerular volumetric density and number of glomeruli of Ischemia were lower than Sham but Ischemia Resveratrol had no difference compared to sham groups.
CONCLUSIONS
Preoperative administration of resveratrol has renoprotective effects, preventing the glomerular number reduction observed in warm ischemia.
背景
本研究旨在定量评估白藜芦醇在肾热缺血期间的保护作用。
方法
大鼠分为 4 组：假手术组、假手术白藜芦醇组、缺血组、缺血白藜芦醇组。假手术白藜芦醇组和缺血白藜芦醇组在术前给予白藜芦醇。缺血组和缺血白藜芦醇组夹闭肾血管。动物在手术后四周处死。测量血清尿素和肌酐。评估肾重和体积、皮质-非皮质面积比、皮质体积、肾小球体积密度、体积加权平均肾小球体积和每肾肾小球数。
结果
与假手术组相比，缺血组血清尿素增加了 10.4%，而缺血白藜芦醇组与假手术组之间无差异。缺血组的肾小球体积密度和肾小球数低于假手术组，但缺血白藜芦醇组与假手术组无差异。
结论
术前给予白藜芦醇具有肾保护作用，可防止热缺血引起的肾小球数量减少。
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