Inoue R
Department of Obstetrics and Gynecology, Asahikawa Medical College.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Dec;40(12):1837-43.
In order to analyze Oncotrophoblast antigen, I used monoclonal antibodies (MoAbs) TM7-3 and TM3-8, which demonstrated a very strong reaction with choriocarcinoma, particularly cytotrophoblast-like tumor cells, in spite of a very weak reaction in normal chorionic villi. I examined their reaction with other tumors of non-trophoblastic origin. Those examined were 58 malignant cases, 2 low-potential malignant cases and 14 benign cases. Immunofluorescence and immunoperoxidase stainings show that 1) TM7-3 and TM3-8 had similar patterns of distribution. 2) In various normal tissues tested, only a part of the urinary tubules of the kidneys and the duct of the pancreas reacted with these MoAbs. 3) In various neoplastic tissues, ovarian serous cancer (3/6), ovarian clear cell cancer (3/3), ovarian undifferentiated cancer (2/2) and well differentiated uterine corpus cancer (3/3) showed a positive reaction with TM7-3 and TM3-8. On the other hand, no uterine cervical squamous cell cancer (0/20) showed a positive reaction. These results suggest that TM7-3 and TM3-8 may be valuable for research on the relationship between the oncotrophoblast antigen and adenocarcinoma of the ovary.
为了分析肿瘤滋养层抗原，我使用了单克隆抗体（MoAbs）TM7 - 3和TM3 - 8，它们与绒毛膜癌，特别是细胞滋养层样肿瘤细胞呈现出非常强烈的反应，尽管在正常绒毛膜绒毛中的反应非常微弱。我检查了它们与其他非滋养层来源肿瘤的反应。所检查的包括58例恶性病例、2例低潜能恶性病例和14例良性病例。免疫荧光和免疫过氧化物酶染色显示：1）TM7 - 3和TM3 - 8具有相似的分布模式。2）在各种测试的正常组织中，只有肾脏的部分肾小管和胰腺导管与这些单克隆抗体发生反应。3）在各种肿瘤组织中，卵巢浆液性癌（3/6）、卵巢透明细胞癌（3/3）、卵巢未分化癌（2/2）和高分化子宫内膜癌（3/3）与TM7 - 3和TM3 - 8呈现阳性反应。另一方面，子宫颈鳞状细胞癌无一例（0/20）呈现阳性反应。这些结果表明，TM7 - 3和TM3 - 8对于研究肿瘤滋养层抗原与卵巢腺癌之间的关系可能具有重要价值。
