Yamashita K, Nakamura T, Shimizu T, Ohno H
Am J Reprod Immunol Microbiol. 1986 Aug;11(4):130-4. doi: 10.1111/j.1600-0897.1986.tb00046.x.
We have established two monoclonal antibodies (TM7-3 and TM3-8) that react to choriocarcinoma cells. Both of these monoclonal antibodies have shown a similar reactive pattern to human cell lines, normal and neoplastic trophoblast tissues, and other fetal and adult tissues. They have reacted to nine of the ten choriocarcinoma cell lines, as well as to Hela cells (a cervical carcinoma cell line). During a cellular radioimmunoassay, neither TM7-3 nor TM3-8 reacted to two T lymphoblastoid cell lines or three B lymphoblastoid cell lines. Immunofluorescence and immunoperoxidase staining showed that both monoclonal antibodies reacted selectively to the cytotrophoblast-like tumor cells of a choriocarcinoma and a hydatidiform mole but not to syncytiotrophoblast-like tumor cells. TM7-3 and TM3-8 also reacted slightly to the normal cytotrophoblast of early human chorionic villi under the same conditions as they did to choriocarcinoma tissues, TM7-3 and TM3-8 bind only to a part of the urinary tubles of the kidney and to the ducts of the pancreas of both adult and fetus.
我们已制备出两种可与绒毛膜癌细胞发生反应的单克隆抗体（TM7 - 3和TM3 - 8）。这两种单克隆抗体对人细胞系、正常及肿瘤性滋养层组织以及其他胎儿和成人组织均呈现出相似的反应模式。它们可与十种绒毛膜癌细胞系中的九种以及Hela细胞（一种宫颈癌细胞系）发生反应。在细胞放射免疫测定中，TM7 - 3和TM3 - 8对两种T淋巴母细胞系或三种B淋巴母细胞系均无反应。免疫荧光和免疫过氧化物酶染色显示，这两种单克隆抗体均选择性地与绒毛膜癌和葡萄胎的细胞滋养层样肿瘤细胞发生反应，而不与合体滋养层样肿瘤细胞发生反应。在与绒毛膜癌组织相同的条件下，TM7 - 3和TM3 - 8对早期人绒毛膜绒毛的正常细胞滋养层也有轻微反应，TM7 - 3和TM3 - 8仅与成人及胎儿肾脏的部分肾小管和胰腺导管结合。
