Zhang Qian, Yang Zai-Fu, Cheng Wei, Wijayawardene Nalin N, Hyde Kevin D, Chen Zhuo, Wang Yong
Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China.
Department of Practaculture Science, Animal Science College, Guizhou University, Guiyang, Guizhou 550025, China.
MycoKeys. 2020 Feb 13;63:49-67. doi: 10.3897/mycokeys.63.49264. eCollection 2020.
Five strains isolated from diseased leaves of lemongrass () in Guangxi Province, China, were examined. NCBI-Blast searches of ITS sequences suggested a high degree of similarity (99-100%) to , , , and . To accurately identify these strains, we further analysed their morphology and phylogenetic relationships based on combinations of ITS, GAPDH, and 1 gene sequences. Morphological observations indicated that the key character differing from similar species was conidial size, whereas phylogenetic analyses indicated that the five strains represent one species that is also distinct from , and by conidial size and conidiophore length. Thus, the strains examined are found to represent a new species described herein as . The pathogenicity test on the host and detached leaves confirmed the new species to be pathogenic on leaves. Standardised requirements for reliable identification of pathogens are also proposed.
对从中国广西省柠檬草病叶中分离出的5个菌株进行了检测。对ITS序列进行NCBI-Blast搜索表明,它们与[具体物种1]、[具体物种2]、[具体物种3]、[具体物种4]和[具体物种5]具有高度相似性(99%-100%)。为了准确鉴定这些菌株,我们基于ITS、GAPDH和[具体基因]基因序列的组合,进一步分析了它们的形态和系统发育关系。形态学观察表明,与相似物种不同的关键特征是分生孢子大小,而系统发育分析表明,这5个菌株代表一个物种,该物种在分生孢子大小和分生孢子梗长度上也与[具体物种1]、[具体物种2]和[具体物种3]不同。因此,所检测的菌株被发现代表本文描述的一个新物种,即[新物种名称]。对寄主和离体叶片的致病性测试证实该新物种对[寄主名称]叶片具有致病性。还提出了可靠鉴定[病原菌名称]病原体的标准化要求。