Kumar Indrajeet, Bhattacharya Jayanta, Das Bidus Kanti, Lahiri Pooja
1School of Environmental Science and Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302 India.
2Department of Mining Engineering, Indian Institute of Technology Kharagpur, Kharagpur, India.
3 Biotech. 2020 Mar;10(3):94. doi: 10.1007/s13205-020-2101-1. Epub 2020 Feb 5.
The supplementation of 50 ppm dosed silver nanoparticles (AgNPs) as a disinfectant in broilers drinking water was investigated to examine their growth performance, blood serum biochemistry, and organ histology in the case group, compared to the control. The growth performance parameters, such as water intake, feed intake, and body weight were recorded 6 times, each in an interval of 7 days, over a period of 42 days. At the end of each 42 days, the blood and major organs of the 1 case boiler out of 75 and 1 control broiler out of 75 were collected in random. The procedure was repeated 3 sets one after another, each consisting 42 day intervening period. The liver enzyme, lipid profile, glucose level, organ histology, and concentration of AgNPs in liver, spleen, heart, and small intestine were determined. The obtained results show that the growth performance of the case broilers was significantly higher than the control section ( < 0.05). However, in all the three sets the changes in lipid profile, liver enzyme, and glucose level of the case broilers were not statistically significantly different compared to the control ( > 0.05). The histology of liver, kidney, heart, spleen, and small intestine of broilers has not shown any damages to the cells as compared to the control samples. In the case samples, the highest concentration of AgNPs was observed in the small intestine (5.44 µg/g) followed by liver (4.32 µg/g), kidney (3.94 µg/g), heart (3.82 µg/g), and spleen (3.49 µg/g). The present study concludes that the administering 50 ppm AgNPs of average 15 nm size in the poultry drinking water was found safe for consumption as well as for growth enhancing, due to better bird comfort.
研究了在肉鸡饮用水中添加50 ppm剂量的银纳米颗粒（AgNPs）作为消毒剂的情况，以检查其在病例组中的生长性能、血清生化指标和器官组织学，并与对照组进行比较。在42天的时间里，每隔7天记录一次生长性能参数，如饮水量、采食量和体重，共记录6次。在每42天结束时，从75只病例肉鸡中随机选取1只，从75只对照肉鸡中随机选取1只，采集血液和主要器官。该过程连续重复3组，每组间隔42天。测定了肝酶、血脂、血糖水平、器官组织学以及肝脏、脾脏、心脏和小肠中AgNPs的浓度。所得结果表明，病例组肉鸡的生长性能显著高于对照组（P<0.05）。然而，在所有三组中，病例组肉鸡的血脂、肝酶和血糖水平变化与对照组相比无统计学显著差异（P>0.05）。与对照样本相比，肉鸡肝脏、肾脏、心脏、脾脏和小肠的组织学未显示细胞有任何损伤。在病例样本中，AgNPs浓度最高的是小肠（5.44 μg/g），其次是肝脏（4.32 μg/g）、肾脏（3.94 μg/g）、心脏（3.82 μg/g）和脾脏（3.49 μg/g）。本研究得出结论，在家禽饮用水中添加平均粒径为15 nm的50 ppm AgNPs，由于能提高家禽舒适度，被认为对食用和生长促进都是安全的。
