Du Liangqing, Gao Boliang, Liang JinFeng, Wang Ya, Xiao Yiwen, Zhu Du
1Key Lab of Bioprocess Engineering of Jiangxi Province, College of life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013 China.
2Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022 China.
3 Biotech. 2020 Mar;10(3):100. doi: 10.1007/s13205-020-2068-y. Epub 2020 Feb 6.
Glycyrrhetinic acid monoglucuronide (GAMG) is a novel and low-calorie sweetener that is widely applied in the food industry. This study aimed to enhance the production of fungal β-d-glucuronidase (GUS) via a novel fermentation technique by evaluating the effects of the various microparticles on DX-THS3 GUS production. Results showed that the silica microparticle greatly affected the morphology of DX-THS3 strain relative to the other microparticles. Microbial structure imaging results showed that the smallest average diameter of fungal pellets was achieved (0.7 ± 0.1 mm) by adding 10 g/L (600 mesh) of silica. The diameter of the control was 3.0 ± 0.5 mm in shake flask fermentation. The GUS activity and biomass of DX-THS3 reached 680 U/mL and 4.2 g/L, respectively, with the use of 10 g/L of silica microparticles, whereas those of the control were 210 U/mL and 2.8 g/L via shake flask fermentation. The findings in this study may provide a potential strategy for designing the morphology of filamentous fungi using microparticles in the industrial production of GAMG.
甘草次酸单葡萄糖醛酸苷（GAMG）是一种新型低热量甜味剂，广泛应用于食品工业。本研究旨在通过评估各种微粒对DX - THS3 β - D - 葡萄糖醛酸酶（GUS）产量的影响，采用新型发酵技术提高真菌β - D - 葡萄糖醛酸酶的产量。结果表明，相对于其他微粒，二氧化硅微粒对DX - THS3菌株的形态有很大影响。微生物结构成像结果显示，添加10 g/L（600目）二氧化硅时，真菌菌球的平均直径最小（0.7±0.1 mm）。在摇瓶发酵中，对照的直径为3.0±0.5 mm。使用10 g/L二氧化硅微粒时，DX - THS3的GUS活性和生物量分别达到680 U/mL和4.2 g/L，而通过摇瓶发酵，对照的GUS活性和生物量分别为210 U/mL和2.8 g/L。本研究结果可能为在GAMG工业生产中使用微粒设计丝状真菌形态提供潜在策略。
