Boerman R H, Peters A C, Verheij M, Raap A K, van Ekdom L T, van Eer A, van der Ploeg M
Department of Neurology, University of Leiden, The Netherlands.
J Neurol Sci. 1988 Nov;87(2-3):245-54. doi: 10.1016/0022-510x(88)90249-3.
Experimentally infected mice were used to assess the value of immunofluorescence (IF) procedures in cerebrospinal fluid (CSF) cells in comparison with routine viral culture of CSF for diagnosis of herpes simplex type-1 (HSV-1) encephalitis. Virus specific antigen was detected by immunofluorescence in the majority of CSF cells in 75% of infected animals. In contrast, only 20% of infected mice had positive viral cultures, which sometimes took as long as a week to show a cytopathological effect (CPE). It is concluded that antigen detection by immunofluorescence is a rapid, specific and sensitive technique for demonstrating HSV-1 antigen in CSF cells of experimentally infected mice. Our results put forward a plea for further studies using these techniques on CSF samples from patients with suspected HSV-1 encephalitis. The prerequisites for reliable interpretation of results have been defined in this study.
使用实验感染的小鼠来评估脑脊液（CSF）细胞中免疫荧光（IF）检测程序相对于CSF常规病毒培养在诊断单纯疱疹病毒1型（HSV-1）脑炎方面的价值。在75%的感染动物中，通过免疫荧光在大多数CSF细胞中检测到病毒特异性抗原。相比之下，只有20%的感染小鼠病毒培养呈阳性，病毒培养有时需要长达一周时间才能显示出细胞病变效应（CPE）。得出结论，免疫荧光抗原检测是一种快速、特异且灵敏的技术，可用于在实验感染小鼠的CSF细胞中检测HSV-1抗原。我们的结果呼吁对疑似HSV-1脑炎患者的CSF样本进一步使用这些技术进行研究。本研究已明确了可靠解读结果的前提条件。
