Institute of Applied Chemistry, Hebei North University, Zhangjiakou 075000, China.
Drug Support Center, Peoples Liberation Army General Hospital, Beijing 100853, China.
Curr Pharm Biotechnol. 2020;21(11):1088-1098. doi: 10.2174/1389201021666200226122742.
The folate-modified graphene oxide (GO-FA), which had good stability and biocompatibility on rat glioma cells was successfully prepared.
The formation and composition of GO-FA were confirmed by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectrum (FT-IR), Raman spectra and X-ray Photoelectron Spectroscopy (XPS spectra). The cell experiment suggested good biocompatibility of GO-FA on rat glioma cells.
The experiment of GO-FA loading with Temozolomide (TMZ) showed that the maximum drug loading of GO-FA was 8.05 ± 0.20 mg/mg, with the drug loading rate of 89.52 ± 0.19 %. When TMZ was released from the folate-modified graphene oxide loading with temozolomide (GO-FATMZ), its release behavior in vitro showed strong pH dependence and sustained release property. The growth of rat glioma cells can be effectively inhibited by GO-FA-TMZ, with the cell inhibition rate as high as 91.72 ± 0.13 % at the concentration of 600 μg/mL and time of 72 h.
According to the above experimental results, this composite carrier has potential applications in drug delivery and cancer therapy.
成功制备了叶酸修饰氧化石墨烯（GO-FA），其在大鼠神经胶质瘤细胞上具有良好的稳定性和生物相容性。
通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）、拉曼光谱和 X 射线光电子能谱（XPS 谱）证实了 GO-FA 的形成和组成。细胞实验表明 GO-FA 对大鼠神经胶质瘤细胞具有良好的生物相容性。
GO-FA 载药实验表明，GO-FA 的最大载药量为 8.05±0.20mg/mg，载药率为 89.52±0.19%。当 TMZ 从载有替莫唑胺的叶酸修饰氧化石墨烯（GO-FATMZ）中释放出来时，其体外释放行为表现出强烈的 pH 依赖性和持续释放特性。GO-FA-TMZ 能有效抑制大鼠神经胶质瘤细胞的生长，在浓度为 600μg/mL、时间为 72h 时，细胞抑制率高达 91.72±0.13%。
根据上述实验结果，该复合载体在药物传递和癌症治疗方面具有潜在的应用价值。
