Research Centre on Animal Cognition, Center for Integrative Biology, CNRS, University of Toulouse, F-31062 Toulouse Cedex 09, France.
Instituto de Biología Celular y Neurociencias (IBCN) "Dr Eduardo De Robertis," CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina; Departamento de Fisiología, Biología Molecular y Celular "Dr. Héctor Maldonado" (FBMC), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Cell Rep. 2020 Feb 25;30(8):2603-2613.e3. doi: 10.1016/j.celrep.2020.01.086.
Research on honeybee memory has led to a widely accepted model in which a single pairing of an odor stimulus with sucrose induces memories that are independent of protein synthesis but is unable to form protein-synthesis-dependent long-term memory (LTM). The latter is said to arise only after three or more pairings of odor and sucrose. Here, we show that this model underestimates the capacity of the bee brain to form LTMs after a unique appetitive experience. Using state-of-the art conditioning setups and individual-based analyses of conditioned responses, we found that protein-synthesis-dependent memories are formed already 4 h after the single conditioning trial and persist even 3 days later. These memories (4 h, 24 h, and 72 h) exhibit different dependencies on transcription and translation processes. Our results thus modify the traditional view of one-trial memories in an insect with a model status for memory research.
对蜜蜂记忆的研究提出了一个被广泛接受的模型，即在单一气味刺激与蔗糖配对的情况下，会产生独立于蛋白质合成的记忆，但无法形成依赖蛋白质合成的长期记忆（LTM）。后者据说只有在三次或更多次气味和蔗糖配对后才会出现。在这里，我们表明，这个模型低估了蜜蜂大脑在独特的食欲体验后形成 LTM 的能力。使用最先进的 Conditioning 设置和基于个体的条件反应分析，我们发现依赖蛋白质合成的记忆在单一 Conditioning 试验后 4 小时就已经形成，并持续到 3 天后。这些记忆（4 小时、24 小时和 72 小时）在转录和翻译过程中有不同的依赖性。因此，我们的研究结果改变了昆虫中单一试记忆的传统观点，为记忆研究提供了一个模型。
