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A20表达下调增强免疫反应和细胞凋亡并减少人呼吸道合胞病毒感染细胞中的病毒产生。

Downregulation of A20 Expression Increases the Immune Response and Apoptosis and Reduces Virus Production in Cells Infected by the Human Respiratory Syncytial Virus.

作者信息

Martín-Vicente María, González-Sanz Rubén, Cuesta Isabel, Monzón Sara, Resino Salvador, Martínez Isidoro

机构信息

Unidad de Infección Viral e Inmunidad. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.

Unidad de Bioinformática. Centro Nacional de Microbiología, Instituto de Salud Carlos III. Majadahonda, 28220 Madrid, Spain.

出版信息

Vaccines (Basel). 2020 Feb 24;8(1):100. doi: 10.3390/vaccines8010100.

DOI:10.3390/vaccines8010100
PMID:32102364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7157707/
Abstract

Human respiratory syncytial virus (HRSV) causes severe lower respiratory tract infections in infants, the elderly, and immunocompromised adults. Regulation of the immune response against HRSV is crucial to limiting virus replication and immunopathology. The A20/TNFAIP3 protein is a negative regulator of nuclear factor kappa B (NF-kB) and interferon regulatory factors 3/7 (IRF3/7), which are key transcription factors involved in the inflammatory/antiviral response of epithelial cells to virus infection. Here, we investigated the impact of A20 downregulation or knockout on HRSV growth and the induction of the immune response in those cells. Cellular infections in which the expression of A20 was silenced by siRNAs or eliminated by gene knockout showed increased inflammatory/antiviral response and reduced virus production. Similar results were obtained when the expression of A20-interacting proteins, such as TAX1BP1 and ABIN1, was silenced. Additionally, downregulation of A20, TAX1BP1, and ABIN1 increased cell apoptosis in HRSV-infected cells. These results show that the downregulation of A20 expression might contribute in the control of HRSV infections by potentiating the early innate immune response and increasing apoptosis in infected cells.

摘要

人呼吸道合胞病毒（HRSV）可导致婴儿、老年人及免疫功能低下的成年人发生严重的下呼吸道感染。针对HRSV的免疫反应调节对于限制病毒复制和免疫病理学至关重要。A20/TNFAIP3蛋白是核因子κB（NF-κB）和干扰素调节因子3/7（IRF3/7）的负调节因子，而NF-κB和IRF3/7是上皮细胞对病毒感染的炎症/抗病毒反应中涉及的关键转录因子。在此，我们研究了A20下调或敲除对HRSV生长以及这些细胞中免疫反应诱导的影响。通过小干扰RNA（siRNA）使A20表达沉默或通过基因敲除消除A20的细胞感染显示出炎症/抗病毒反应增强且病毒产生减少。当A20相互作用蛋白（如TAX1BP1和ABIN1）的表达沉默时，也获得了类似的结果。此外，A20、TAX1BP1和ABIN1的下调增加了HRSV感染细胞中的细胞凋亡。这些结果表明，A20表达的下调可能通过增强早期先天免疫反应和增加感染细胞中的凋亡来有助于控制HRSV感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/bb906661bcda/vaccines-08-00100-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/7c0647613293/vaccines-08-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/713434622165/vaccines-08-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/1738b4a17249/vaccines-08-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/7742182dea1a/vaccines-08-00100-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/53256da9b730/vaccines-08-00100-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/bb906661bcda/vaccines-08-00100-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/7c0647613293/vaccines-08-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/713434622165/vaccines-08-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/1738b4a17249/vaccines-08-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/7742182dea1a/vaccines-08-00100-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/53256da9b730/vaccines-08-00100-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e8a/7157707/bb906661bcda/vaccines-08-00100-g006.jpg

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2
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5
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