ter Welle H F, Baartscheer A, Fiolet J W, Schumacher C A
Department of Clinical and Experimental Cardiology, University Hospital, Academisch Medisch Centrum, Amsterdam, The Netherlands.
J Mol Cell Cardiol. 1988 May;20(5):435-41. doi: 10.1016/s0022-2828(88)80135-4.
The relationship between the percentage of rod-shaped rat heart myocytes and ATP, creatine phosphate, creatine and inorganic phosphate content was determined. With these values the free energy of ATP hydrolysis was calculated and found to be 59.2 kJ/mol, a much higher value than found for the perfused rat heart. When, during the isolation procedure, creatine was present in the perfusion medium during the low-calcium period, the total creatine content of the myocytes after isolation was comparable to that found in the perfused rat heart. However, when creatine was absent during this low-calcium perfusion period, total creatine content of the myocytes was significantly lower. This difference is caused by leakage of creatine from healthy cells. The free energy of ATP hydrolysis was not affected by the absence of creatine during the low-calcium perfusion period.
测定了杆状大鼠心肌细胞百分比与三磷酸腺苷（ATP）、磷酸肌酸、肌酸和无机磷酸盐含量之间的关系。利用这些数值计算出ATP水解的自由能为59.2千焦/摩尔，这一数值远高于灌注大鼠心脏中的测定值。在分离过程中，当低钙期灌注培养基中存在肌酸时，分离后心肌细胞的总肌酸含量与灌注大鼠心脏中的相当。然而，当低钙灌注期不存在肌酸时，心肌细胞的总肌酸含量显著降低。这种差异是由肌酸从健康细胞中泄漏所致。低钙灌注期不存在肌酸对ATP水解的自由能没有影响。
