Kim Seong Min, Vetrivel Preethi, Kim Hun Hwan, Ha Sang Eun, Saralamma Venu Venkatarame Gowda, Kim Gon Sup
Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongsang 52828, Republic of Korea.
Exp Ther Med. 2020 Mar;19(3):2161-2170. doi: 10.3892/etm.2020.8472. Epub 2020 Jan 27.
Inflammatory diseases are an important health concern and have a growing incidence worldwide. Thus, developing novel and safe drugs to treat these disorders remains an important pursuit. , locally known as Dowijigi (DJ), is a perennial herb found primarily in Korea and is used to treat various diseases such as hepatitis, inflammation and immune disorders. In the present study, the anti-inflammatory effects of a polyphenolic extract from the DJ flower (PDJ) in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells were investigated. Cell cytotoxicity was assessed using the MTT assay. The production of nitric oxide (NO) and prostaglandin E (PGE) was measured by Griess and ELISA analysis, respectively. The expression levels of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX2) were examined by western blot analysis. Reverse transcription-quantitative PCR was performed to detect the mRNA expression levels of pro-inflammatory cytokines, including tumor necrosis factor α (TNFα), interleukin (IL)-6 and IL-1β, as well as COX2 and iNOS. The production of NO and PGE was significantly decreased following treatment with PDJ. The mRNA expression levels of TNFα, IL-6, IL-1β, COX2 and iNOS were significantly decreased in LPS-induced PDJ co-treated cells compared with the group treated with LPS alone. Western blot analysis indicated that PDJ downregulated the LPS-induced expression of iNOS and COX2, as well as the expression of NF-κB proteins. In conclusion, the present study demonstrated that PDJ exerted anti-inflammatory effects in LPS-induced macrophage cells by suppressing the NF-κB signaling pathway. Therefore, PDJ may be used as a potential therapeutic agent in inflammation.
炎症性疾病是一个重要的健康问题，在全球范围内发病率不断上升。因此，开发新型安全的药物来治疗这些疾病仍然是一项重要的追求。一种当地称为Dowijigi（DJ）的多年生草本植物，主要生长在韩国，用于治疗各种疾病，如肝炎、炎症和免疫紊乱。在本研究中，研究了DJ花多酚提取物（PDJ）对脂多糖（LPS）刺激的小鼠巨噬细胞RAW264.7细胞的抗炎作用。使用MTT法评估细胞毒性。分别通过Griess法和ELISA分析法测量一氧化氮（NO）和前列腺素E（PGE）的产生。通过蛋白质印迹分析检测诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX2）的表达水平。进行逆转录定量PCR以检测促炎细胞因子的mRNA表达水平，包括肿瘤坏死因子α（TNFα）、白细胞介素（IL）-6和IL-1β，以及COX2和iNOS。用PDJ处理后，NO和PGE的产生显著降低。与单独用LPS处理的组相比，在LPS诱导的PDJ共同处理的细胞中，TNFα、IL-6、IL-1β、COX2和iNOS的mRNA表达水平显著降低。蛋白质印迹分析表明，PDJ下调了LPS诱导的iNOS和COX2的表达以及NF-κB蛋白的表达。总之，本研究表明，PDJ通过抑制NF-κB信号通路在LPS诱导的巨噬细胞中发挥抗炎作用。因此，PDJ可能用作炎症的潜在治疗剂。
