电针促进神经干细胞生长。
Electroacupuncture to Increase Neuronal Stem Cell Growth.
作者信息
Dubrovsky Genia, Ha Don, Thomas Anne-Laure, Zhu Minji, Hubacher John, Itoh Tatsuo, Dunn James C Y
机构信息
Division of Pediatric Surgery, Department of Surgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA.
Santa Monica Acupuncture Center, Los Angeles, CA.
出版信息
Med Acupunct. 2020 Feb 1;32(1):16-23. doi: 10.1089/acu.2019.1381. Epub 2020 Feb 3.
Neuropathic intestinal disorders continue to pose a significant burden, and current treatment options do not target the underlying cellular deficiencies. The goal of this study is to determine whether acupuncture and electroacupuncture (EA) can affect the growth of neuronal cells. Three groups of Lewis rats received 25 minutes of acupuncture twice a week for 10 weeks. The 3 groups of rats received treatment with either sham acupuncture (SA), real acupuncture (RA), or EA. After 10 weeks of treatment, skin and intestinal tissue were collected and analyzed for histology and mRNA expression of neuronal marker genes. Compared with rats that received SA, rats that received RA and EA showed a significant increase in the mRNA expression levels of multiple neuronal genes in the skin. No significant histologic changes were seen. Acupuncture and EA result in significant changes in the expression of genes implicated as markers for neural stem cells, neural cell development, and neurons. This may, therefore, provide a novel avenue for developing treatments in patients suffering from intestinal aganglionic and neuropathic diseases.
神经性肠道疾病仍然构成重大负担，而目前的治疗方案并未针对潜在的细胞缺陷。本研究的目的是确定针刺和电针（EA）是否会影响神经元细胞的生长。三组Lewis大鼠每周接受两次25分钟的针刺治疗，持续10周。三组大鼠分别接受假针刺（SA）、真针刺（RA）或电针治疗。治疗10周后，收集皮肤和肠道组织，分析其组织学和神经元标记基因的mRNA表达。与接受假针刺的大鼠相比，接受真针刺和电针的大鼠皮肤中多个神经元基因的mRNA表达水平显著增加。未见明显的组织学变化。针刺和电针导致与神经干细胞、神经细胞发育和神经元标记相关的基因表达发生显著变化。因此，这可能为开发治疗肠道神经节缺失和神经性疾病患者的新方法提供一条途径。
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